Abstract | BACKGROUND AND PURPOSE: METHODS: We administered PACAP38 as an intravenous bolus (20 nmol/kg body wt) followed by an intravenous infusion for 48 hours using a micro-osmotic pump at a rate of 160 pmol/microL per hour, beginning 4, 8, or 12 hours after a 2-hour transient MCAO using a filament model. The size of the infarct was determined by examining 2-mm-thick brain sections stained with triphenyltetrazolium chloride, followed by image analysis. Control animals received intravenously 0.1% bovine serum albumin in 0.9% saline as a bolus and infusion at the same time intervals. RESULTS: The administration of PACAP38 beginning 4 hours after MCAO significantly reduced the infarct size by 50.88%. Treatment with PACAP38 starting 8 or 12 hours after the onset of ischemia did not result in a significant reduction of the infarct size, although infarct volumes tended to be smaller than in the control groups. CONCLUSIONS: Systemic administration of PACAP38 should be clinically useful for reducing brain damage resulting from stroke even when administration is delayed for several hours.
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Authors | D Reglodi, A Somogyvari-Vigh, S Vigh, T Kozicz, A Arimura |
Journal | Stroke
(Stroke)
Vol. 31
Issue 6
Pg. 1411-7
(Jun 2000)
ISSN: 0039-2499 [Print] United States |
PMID | 10835464
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adcyap1 protein, rat
- Neuropeptides
- Neuroprotective Agents
- Pituitary Adenylate Cyclase-Activating Polypeptide
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Topics |
- Animals
- Brain Damage, Chronic
(etiology, pathology, prevention & control)
- Drug Administration Schedule
- Drug Evaluation, Preclinical
- Infarction, Middle Cerebral Artery
(complications, drug therapy, pathology)
- Injections, Intravenous
- Ischemic Attack, Transient
(drug therapy, etiology, pathology)
- Male
- Neuropeptides
(administration & dosage, therapeutic use)
- Neuroprotective Agents
(administration & dosage, therapeutic use)
- Pituitary Adenylate Cyclase-Activating Polypeptide
- Rats
- Time Factors
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