Abstract |
The Yoshida AH-130 rat ascites hepatoma is a model system for studying the mechanisms involved in the protein hypercatabolism associated with cancer cachexia. The present study was aimed at investigating if the calpain-3 gene expression in skeletal muscle was affected by tumor growth. The results presented clearly show that calpain-3 gene expression is considerably reduced in experimental cancer cachexia, while there is a reciprocal change in the expression of the ubiquitin-dependent proteolytic system and in the ubiquitous m-calpain. The results, observed during cancer cachexia, suggest a potential counterregulatory role of calpain-3 in muscle proteolysis.
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Authors | S Busquets, C García-Martínez, B Alvarez, N Carbó, F J López-Soriano, J M Argilés |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1475
Issue 1
Pg. 5-9
(Jun 01 2000)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 10806331
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA Probes
- Muscle Proteins
- RNA
- Calpain
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Topics |
- Animals
- Cachexia
(enzymology, etiology)
- Calpain
(genetics, metabolism)
- DNA Probes
- Down-Regulation
- Gene Expression Regulation, Enzymologic
- Male
- Muscle Proteins
(genetics, metabolism)
- Muscle, Skeletal
(enzymology)
- Neoplasms, Experimental
(complications)
- RNA
(isolation & purification)
- Rats
- Rats, Wistar
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