Abstract |
The changes in human red blood cell microrheology in different glutaraldehyde (3.0 and 5.0 x 10(-6) mol x l(-1)) and exaprolol (2.5 and 5.0 x 10(-4) mol x l(-1)) concentrations were studied. The method of millipore filtration was compared with the method of cation-osmotic hemolysis. Both drugs prolonged the filtration time. Cation-osmotic hemolysis in glutaraldehyde-treated cells was significantly lower in comparison with the control group. On the other hand, there was a significant increase in cation-osmotic hemolysis in exaprolol-treated cells. Besides cation-osmotic hemolysis and filterability of erythrocytes, we evaluated the medium cell volume (MCV) and the medium cell hemoglobin concentration (MCHC). No changes in MCV and MCHC in glutaraldehyde-treated cells were observed. However, the MCV was significantly lower and the MCHC was significantly higher in exaprolol-treated cells. In conclusion, we suggest that the method of cation-osmotic hemolysis is more sensitive than the filtration method for determination of red blood cell microrheology.
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Authors | J Mojzis, A Nicák, A Linková, M Jandoseková, L Mirossay |
Journal | Physiological research
(Physiol Res)
Vol. 48
Issue 6
Pg. 411-6
( 1999)
ISSN: 0862-8408 [Print] Czech Republic |
PMID | 10783905
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Adrenergic beta-Antagonists
- Cations
- Disinfectants
- Propanolamines
- exaprolol
- Glutaral
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Topics |
- Adrenergic beta-Antagonists
(pharmacology)
- Adult
- Cations
(pharmacology)
- Disinfectants
(pharmacology)
- Erythrocyte Deformability
(drug effects, physiology)
- Glutaral
(pharmacology)
- Hemolysis
(drug effects, physiology)
- Humans
- Middle Aged
- Osmotic Pressure
- Propanolamines
(pharmacology)
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