Abstract |
Neurothelin/HT7, a transmembrane glycoprotein of the immunoglobulin superfamily, is a marker of blood-brain barrier (BBB)-forming endothelial cells. We have studied the expression of neurothelin in tumors grown on the chorioallantoic membrane (CAM) of chick embryos. We inoculated each 3-5 x 10(6) rat C6 glioma, rat 10AS pancreatic carcinoma, human A375 melanoma, and human mammary duct adenoma cells on the CAM of 10-day-old chick embryos. The tumors were harvested on day 17. All four tumor cell lines formed solid tumors which were supplied by vessels of CAM origin. Foci of bleeding were regularly observed within the tumors. All four tumors induced the expression of neurothelin/HT7 (but not of glucose transporter-1) in tumor endothelial cells, whereas expression in adjacent endothelial cells of normal CAM did not occur. Confocal laser scanning microscopy revealed that the pattern of neurothelin expression in tumor endothelial cells was different from that in normal central nervous system (CNS) endothelium, but the relative molecular weight of neurothelin, studied by western blot analysis, was the same in brain and in tumors. It has been shown that, with increasing malignancy, vessels of CNS tumors lose their morphological characteristics, and BBB markers such as the glucose transporter-1 are downregulated. Our results show that, in contrast, the BBB marker, neurothelin, is expressed de novo in tumor endothelial cells. Potential common functions of neurothelin in endothelial cells of the CNS and tumors are discussed.
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Authors | M Papoutsi, H Kurz, C Schächtele, D Marmé, B Christ, F Pröls, J Wilting |
Journal | Histochemistry and cell biology
(Histochem Cell Biol)
Vol. 113
Issue 2
Pg. 105-13
(Feb 2000)
ISSN: 0948-6143 [Print] Germany |
PMID | 10766263
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD
- Antigens, Neoplasm
- Antigens, Surface
- Avian Proteins
- BSG protein, human
- Biomarkers
- Biomarkers, Tumor
- Blood Proteins
- Bsg protein, Gallus gallus
- Bsg protein, rat
- Glucose Transporter Type 1
- Membrane Glycoproteins
- Monosaccharide Transport Proteins
- Neoplasm Proteins
- SLC2A1 protein, human
- Slc2a1 protein, rat
- Basigin
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Topics |
- Allantois
- Animals
- Antigens, CD
- Antigens, Neoplasm
- Antigens, Surface
- Avian Proteins
- Basigin
- Biomarkers
- Biomarkers, Tumor
(immunology)
- Blood Proteins
- Blood-Brain Barrier
- Brain
(blood supply)
- Chick Embryo
- Chorion
- Embryonic Induction
- Endothelium
(chemistry, cytology, embryology)
- Endothelium, Vascular
(chemistry)
- Gene Expression Regulation
- Glucose Transporter Type 1
- Humans
- Immunoblotting
- Membrane Glycoproteins
(biosynthesis, genetics)
- Microcirculation
- Microscopy, Confocal
- Monosaccharide Transport Proteins
(immunology)
- Neoplasm Proteins
(biosynthesis, genetics)
- Neoplasm Transplantation
- Neoplasms, Experimental
(blood supply, chemistry)
- Rats
- Time Factors
- Tumor Cells, Cultured
(transplantation)
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