Hematoxylin and safranin O staining of frozen sections.

Abstract

Currently the hematoxylin and eosin staining procedure is the most popular among Mohs surgeons for histology. However, safranin O, a cheaper and relatively safer stain which is predominantly used for plant histology, should be considered as it offers similar or improved accuracy in the diagnosis of frozen sections of basal and squamous cell carcinomas.

• Authors: D Tran, M Golick, H Rabinovitz, D Rivlin, G Elgart, B Nordlow
• Journal: Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.] (Dermatol Surg) Vol. 26 Issue 3 Pg. 197-9 (Mar 2000) ISSN: 1076-0512 [Print] United States
• PMID: 10759792 (Publication Type: Journal Article)

Chemical References

• Coloring Agents
• Phenazines
• safranine T
• Hematoxylin

Topics

• Carcinoma, Basal Cell (diagnosis)
• Carcinoma, Squamous Cell (diagnosis)
• Coloring Agents
• Cytodiagnosis
• Frozen Sections
• Hematoxylin
• Humans
• Mohs Surgery
• Phenazines
• Skin Neoplasms (diagnosis, surgery)