[Clinical implications of a new TSH receptor antibody assay (DYNOtest TRAKhuman) in autoimmune thyroid diseases].

Abstract

AIM:

Conventional radioreceptor-antibody-assays (RAAs) fail in the detection of TSH-receptor antibodies (TRAKs) in 10-30% of patients with Graves' disease (GD). The aim of this study was the evaluation of the diagnostic and clinical impact of a new RRA (DYNOtest TRAKhuman) which uses the human recombinant TSH-Receptor in the diagnosis of autoimmune thyroid disease.

METHODS:

Sera from 142 consecutive patients (GD: n = 50, autoimmune thyroiditis/AIT: n = 92) and from 55 controls (31 patients without any thyroid disease and 14 with euthyroid goiter) were evaluated both with the DYNOtest TRAKhuman-assay and a conventional RRA (TRAK-Assay). Thyroid in vitro parameters and thyroid sonography were performed in all patients.

RESULTS:

The DYNOtest TRAK-assay was significantly superior to the conventional RRA in the diagnosis of GD (p < 0.00012), especially in those who were treated by thionamides (p < 0.003) and in the diagnosis of TRAK-positive patients with AIT (p < 0.003). The majority of TRAK-positive AIT-patients suffered from hypothyroidism. One false positive result in patients with euthyroid goiter was found in the TRAK-Assay as well as in the DYNOtest TRAKhuman-Assay. Therefore the specificity of the DYNOtest TRAKhuman was not inferior compared with the conventional assay.

CONCLUSION:

The DYNOtest TRAK-assay is superior in the diagnostic work up of Graves' disease compared with a conventional TRAK-assay and offers an equal specificity.

• Authors: J Meller, I Schreivogel, A Bergmann, N Morgenthaler, M Hüfner, W Becker
• Journal: Nuklearmedizin. Nuclear medicine (Nuklearmedizin) Vol. 39 Issue 1 Pg. 14-8 ( 2000) ISSN: 0029-5566 [Print] Germany
• Vernacular Title: Klinische Implikationen eines neuen TSH-Rezeptor-Antikörper-Assays (DYNOtest TRAKhuman) bei autoimmunen Schilddrüsenerkrankungen.
• PMID: 10726252 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Immunoglobulins, Thyroid-Stimulating
• Reagent Kits, Diagnostic
• Receptors, Thyrotropin
• Recombinant Proteins

Topics

• Female
• Goiter (blood, immunology)
• Graves Disease (blood, immunology)
• Humans
• Immunoglobulins, Thyroid-Stimulating (blood)
• Male
• Middle Aged
• Radioimmunoassay (methods)
• Reagent Kits, Diagnostic
• Receptors, Thyrotropin (immunology)
• Recombinant Proteins (immunology)
• Reference Values