An extensive blood loss activates generalized inflammatory response. Abdominal organs and especially intestines are very sensitive to the
ischemia-reperfusion insults due to
hemorrhagic shock (HS) and blood volume restoration. Previously obtained results suggest that studies on peritoneal lavage fluid (PLF) can contribute to elucidation of inflammatory processes in abdominal organs in HS.
Histamine (H) levels, total cell, and mast cell (MC) numbers, and MC ultrastructure in the fluid lavaged from peritoneal cavity were compared in the following groups of rats: control (gr. 1),
sham operation (gr. 2), untreated
hemorrhagic shock (gr. 3),
shock treated with blood volume restoration with
lactated Ringer's solution (LR) (gr. 4),
shock treated with
platelet activating factor (PAF)-receptor antagonist
Ginkgolide B (BN52021), and LR (gr. 5). A
shock-related significant increase in total cell numbers, MC numbers, MC degranulation, and
histamine levels in PLF were observed. The restoration of blood volume caused further elevation of the above phenomena (gr. 4) while BN52021 seemed to inhibit peritoneal MC mobilization and degranulation as well as to attenuate increase in peritoneal H level (gr. 5). The peritoneal cavity is a place of rapid and strong reaction to
hemorrhage. Evaluation of peritoneal
histamine levels might be helpful in the monitoring of
shock dependent intra-abdominal processes. Peritoneal MC mobilization and degranulation, and increase in
histamine level is inhibited by BN52021.