Abstract |
The lack of any markers for oligodendrocyte precursors that can be visualized within the intact CNS is a significant barrier to trials of transplantation of these cells which aim to enhance remyelination in multiple sclerosis. We have therefore asked whether dextran-coated superparamagnetic iron oxide ( SPIO) can be used to label cells prior to transplantation and then visualized within the brain using MRI. We have shown that an oligodendrocyte precursor cell line CG-4 will take up dextran-coated SPIO particles in vitro. The label remains within the cells after transplantation into adult rat brain, as assessed by electron microscopy, and is visible by MRI as a reduction in signal intensity at the transplant site at both 1 and 7 days after surgery. We conclude that MRI detection of SPIO-labelled cells represents a promising and novel approach to the analysis of oligodendroglial cell behaviour following transplantation that has very significant advantages over currently available methods.
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Authors | R J Franklin, K L Blaschuk, M C Bearchell, L L Prestoz, A Setzu, K M Brindle, C ffrench-Constant |
Journal | Neuroreport
(Neuroreport)
Vol. 10
Issue 18
Pg. 3961-5
(Dec 16 1999)
ISSN: 0959-4965 [Print] England |
PMID | 10716241
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Contrast Media
- Dextrans
- Magnetite Nanoparticles
- Oxides
- Iron
- ferumoxides
- Ferrosoferric Oxide
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Topics |
- Animals
- Brain
(physiology, ultrastructure)
- Cell Line
- Contrast Media
(pharmacokinetics)
- Dextrans
- Ferrosoferric Oxide
- Iron
(pharmacokinetics)
- Magnetic Resonance Imaging
- Magnetite Nanoparticles
- Microscopy, Electron
- Oligodendroglia
(metabolism, transplantation, ultrastructure)
- Oxides
(pharmacokinetics)
- Rats
- Rats, Sprague-Dawley
- Stem Cell Transplantation
- Stem Cells
(metabolism, ultrastructure)
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