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A monoclonal antibody to ICP4 of MDV recognizing ICP4 of serotype 1 and 3 MDV strains.

Abstract
Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidney cells as antigens. One of the MAbs, 5H8, recognized an epitope toward the carboxyl terminus of ICP4 based on staining of reticuloendotheliosis virus-transformed cells transfected with full-length and truncated ICP4 constructs. This MAb recognized ICP4 in chicken embryo fibroblasts (CEFs) infected with MDV strains JM16 and HVT but not with SB-1 strain. Using Western blot analysis a protein of 155 kDa was detected in CEFs infected with JM16 and HVT strains.
AuthorsZ Xing, Q Xie, R W Morgan, K A Schat
JournalActa virologica (Acta Virol) 1999 Apr-Jun Vol. 43 Issue 2-3 Pg. 113-20 ISSN: 0001-723X [Print] Slovakia
PMID10696430 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Nuclear Proteins
  • Recombinant Proteins
  • Trans-Activators
  • Viral Proteins
  • ICP4 protein, Gallid herpesvirus 2
Topics
  • Animals
  • Antibodies, Monoclonal (biosynthesis, immunology)
  • Antibodies, Viral (biosynthesis, immunology)
  • Blotting, Western
  • Cell Line
  • Chick Embryo
  • Fluorescent Antibody Technique, Indirect
  • Herpesvirus 2, Gallid (classification, immunology)
  • Hybridomas
  • Immunization
  • Lymphocytes (cytology, virology)
  • Mice
  • Mice, Inbred BALB C
  • Nuclear Proteins (analysis, chemistry, immunology)
  • Nucleopolyhedroviruses (genetics, metabolism)
  • Recombinant Proteins (genetics, immunology)
  • Serotyping
  • Trans-Activators (analysis, chemistry, immunology)
  • Transfection
  • Viral Proteins

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