Abstract |
Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidney cells as antigens. One of the MAbs, 5H8, recognized an epitope toward the carboxyl terminus of ICP4 based on staining of reticuloendotheliosis virus-transformed cells transfected with full-length and truncated ICP4 constructs. This MAb recognized ICP4 in chicken embryo fibroblasts (CEFs) infected with MDV strains JM16 and HVT but not with SB-1 strain. Using Western blot analysis a protein of 155 kDa was detected in CEFs infected with JM16 and HVT strains.
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Authors | Z Xing, Q Xie, R W Morgan, K A Schat |
Journal | Acta virologica
(Acta Virol)
1999 Apr-Jun
Vol. 43
Issue 2-3
Pg. 113-20
ISSN: 0001-723X [Print] Slovakia |
PMID | 10696430
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antibodies, Viral
- Nuclear Proteins
- Recombinant Proteins
- Trans-Activators
- Viral Proteins
- ICP4 protein, Gallid herpesvirus 2
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Topics |
- Animals
- Antibodies, Monoclonal
(biosynthesis, immunology)
- Antibodies, Viral
(biosynthesis, immunology)
- Blotting, Western
- Cell Line
- Chick Embryo
- Fluorescent Antibody Technique, Indirect
- Herpesvirus 2, Gallid
(classification, immunology)
- Hybridomas
- Immunization
- Lymphocytes
(cytology, virology)
- Mice
- Mice, Inbred BALB C
- Nuclear Proteins
(analysis, chemistry, immunology)
- Nucleopolyhedroviruses
(genetics, metabolism)
- Recombinant Proteins
(genetics, immunology)
- Serotyping
- Trans-Activators
(analysis, chemistry, immunology)
- Transfection
- Viral Proteins
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