Abstract |
GD1alpha ganglioside-replica peptides were recently isolated from a phage-displayed random pentadecapeptide library by assaying for inhibition of adhesion of RAW117-H10 lymphosarcoma cells to hepatic sinusoidal microvessel endothelial (HSE) cells. We show here that the Trp-His-Trp (WHW) peptide was identified as a minimal sequence of the GD1alpha-replica peptide WHWRHRIPLQLAAGR. The addition of WHW peptide-attached liposomes displayed efficient inhibition of liver metastasis of RAW117-H10 cells as well as of GD1alpha-mediated adhesion of RAW117-H10 cells to HSE cells in vitro. These results suggest that engineered liposomes for peptide delivery are applicable to treatment for metastasis.
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Authors | M Takikawa, H Kikkawa, T Asai, N Yamaguchi, D Ishikawa, M Tanaka, K Ogino, T Taki, N Oku |
Journal | FEBS letters
(FEBS Lett)
Vol. 466
Issue 2-3
Pg. 381-4
(Jan 28 2000)
ISSN: 0014-5793 [Print] England |
PMID | 10682865
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drug Carriers
- GD1alpha-replica peptide
- Liposomes
- Peptides
- Proteins
- ganglioside GD1alpha
- G(M1) Ganglioside
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Topics |
- Amino Acid Sequence
- Animals
- Cell Adhesion
(drug effects)
- Drug Carriers
- G(M1) Ganglioside
(analogs & derivatives, antagonists & inhibitors)
- Liposomes
- Liver Neoplasms, Experimental
(drug therapy, secondary)
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Neoplasm Metastasis
- Peptides
(pharmacology)
- Proteins
(pharmacology)
- Tumor Cells, Cultured
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