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[Effect of recombinant bactericidal/permeability-increasing protein on pulmonary cytokine mRNA expression in rats following hemorrhage and resuscitation].

Abstract
To determine the possible mechanisms underlying beneficial effect of recombinant bactericidal/permeability-increasing protein (rBPI) on acute lung injury response to blood loss, we used reverse transcription polymerase chain reaction to measure pulmonary tumor necrosis factor (TNF), interleukin 6 (IL-6) mRNA expression in a rat model of prolonged hemorrhagic shock (4.00 kPa, 180 min) followed by adequate resuscitation. The results showed that systemic plasma endotoxin concentrations elevated rapidly after a 180-min hemorrhagic insult (P < 0.05), and TNF, IL-6 mRNA expression in the lung were significantly increased at 2, 8 hours after resuscitation respectively. However, treatment with rBPI resulted in almost neutralization of plasma endotoxin values, remarkable reduction of TNF, IL-6 mRNA levels following hemorrhage/resuscitation. Also, it was found that rBPI administration markedly blunted the increase in pulmonary Evans blue dye extravasation, concomitant with a significant decrease in lung myeloperoxidase activity compared with the control group (P < 0.05-0.01). These data suggest that local proinflammatory cytokine mRNA expression associated with gut origin endotoxemia may be an important mechanism contributing to the development of hemorrhage-induced lung injury. Treatment with rBPI is effective in inhibiting marked TNF, IL-6 mRNA expression and ameliorating acute lung injury secondary to severe hemorrhagic shock.
AuthorsY Yao, Z Sheng, Z Shi
JournalZhonghua wai ke za zhi [Chinese journal of surgery] (Zhonghua Wai Ke Za Zhi) Vol. 35 Issue 7 Pg. 389-91 (Jul 1997) ISSN: 0529-5815 [Print] China
PMID10677969 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Infective Agents
  • Antimicrobial Cationic Peptides
  • Blood Proteins
  • Endotoxins
  • Interleukin-6
  • Membrane Proteins
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • bactericidal permeability increasing protein
Topics
  • Animals
  • Anti-Infective Agents (pharmacology)
  • Antimicrobial Cationic Peptides
  • Blood Proteins (pharmacology)
  • Endotoxins (blood)
  • Interleukin-6 (biosynthesis, genetics)
  • Lung (metabolism)
  • Male
  • Membrane Proteins
  • RNA, Messenger (biosynthesis, genetics)
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Shock, Hemorrhagic (metabolism)
  • Tumor Necrosis Factor-alpha (biosynthesis, genetics)

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