Abstract |
A novel receptor for oxidized low-density lipoprotein ( OxLDL), lectin-like OxLDL receptor (LOX-1), was cloned from endothelial cells. Since OxLDL is taken up by vascular smooth muscle cells (VSMC) in atheroma, we analyzed the inducible expression of LOX-1 in VSMC in the present study. Incubation of cultured bovine VSMC with lysophosphatidylcholine (LPC), an atherogenic component of OxLDL, increased the level of mRNA for LOX-1 in a dose- and time-dependent manner. Since LPC did not significantly change the half-life of LOX-1 mRNA, the induction seemed to occur at the transcriptional level. The induction accompanied an increase in the protein level of LOX-1 and activity of OxLDL uptake. Blocking antibody against LOX-1 significantly suppressed the enhanced uptake of OxLDL. Thus, LOX-1 is a major receptor for OxLDL in VSMC as in endothelial cells. The enhanced expression of LOX-1 by LPC suggests that OxLDL and LPC would progressively change the function of VSMC and accelerate atherogenesis in vivo.
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Authors | T Aoyama, M Chen, H Fujiwara, T Masaki, T Sawamura |
Journal | FEBS letters
(FEBS Lett)
Vol. 467
Issue 2-3
Pg. 217-20
(Feb 11 2000)
ISSN: 0014-5793 [Print] England |
PMID | 10675541
(Publication Type: Journal Article)
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Chemical References |
- Lipoproteins, LDL
- Lysophosphatidylcholines
- RNA, Messenger
- Receptors, LDL
- Receptors, Oxidized LDL
- oxidized low density lipoprotein
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Topics |
- Animals
- Arteriosclerosis
(metabolism)
- Cattle
- Cells, Cultured
- Lipoproteins, LDL
(metabolism)
- Lysophosphatidylcholines
(pharmacology)
- Muscle, Smooth, Vascular
(drug effects, metabolism)
- Oxidation-Reduction
- RNA, Messenger
(metabolism)
- Receptors, LDL
(genetics, metabolism)
- Receptors, Oxidized LDL
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