Cyclooxygenase activity in chondrocytes from osteoarthritic and healthy cartilage.

Abstract

OBJECTIVES:

To compare prostaglandin production and cyclooxygenase activity in high-density primary cultures of chondrocytes from patients with osteoarthritis and from healthy controls.

METHODS:

Chondrocytes harvested from operative specimens obtained during hip or knee arthroplasty were cultured at a very high density for 48 h. Cyclooxygenase activity was evaluated based on prostaglandin E2 levels (radioimmunological assay) after addition of arachidonic acid. The chondrocytes were cultured with and without IL-1 beta and TNF alpha.

RESULTS:

Prostaglandin E2 production and cyclooxygenase activity were significantly higher in cultures of chondrocytes from osteoarthritic than from healthy joints. Adding IL-1 beta to the osteoarthritic chondrocyte cultures further increased the high cyclooxygenase activity, whereas adding TNF alpha alone had no effect.

CONCLUSION:

Cultured chondrocytes from osteoarthritic joints showed increased prostaglandin E2 production and cyclooxygenase activity.

• Authors: C Jacques, A Sautet, M Moldovan, B Thomas, L Humbert, F Berenbaum
• Journal: Revue du rhumatisme (English ed.) (Rev Rhum Engl Ed) Vol. 66 Issue 12 Pg. 701-4 (Dec 1999) ISSN: 1169-8446 [Print] France
• PMID: 10649604 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Prostaglandin-Endoperoxide Synthases

Topics

• Cartilage (enzymology)
• Chondrocytes (enzymology)
• Humans
• Osteoarthritis (enzymology)
• Prostaglandin-Endoperoxide Synthases (metabolism)