Generation of protective immune responses to plague by mucosal administration of microsphere coencapsulated recombinant subunits.

Abstract

We have investigated noninvasive immunization to plague. Recombinant subunit antigens, F1 and V from Yersinia pestis, were coencapsulated in biodegradable poly(L100 LD(50's) inhalational challenge with virulent Y. pestis. These data expand on previous findings from our laboratories, providing further insight into the mechanics of safeguarding mice from plague through nasal immunization. Further, these results demonstrate that in a murine model, solid protection from pneumonic plague can be engendered by two intranasal administrations of appropriately formulated recombinant proteins.

• Authors: J E Eyles, E D Williamson, I D Spiers, A J Stagg, S M Jones, H O Alpar
• Journal: Journal of controlled release : official journal of the Controlled Release Society (J Control Release) Vol. 63 Issue 1-2 Pg. 191-200 (Jan 03 2000) ISSN: 0168-3659 [Print] Netherlands
• PMID: 10640592 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Proteins
• Bacterial Vaccines
• Biocompatible Materials
• Immunoglobulin G
• LcrV protein, Yersinia
• Polyesters
• Pore Forming Cytotoxic Proteins
• Vaccines, Synthetic
• caf1 protein, Yersinia pestis
• poly(lactide)

Topics

• Administration, Intranasal
• Animals
• Antibodies, Bacterial (blood)
• Antigens, Bacterial (administration & dosage, genetics, immunology)
• Bacterial Proteins (administration & dosage, genetics, immunology)
• Bacterial Vaccines (administration & dosage, genetics, immunology)
• Biocompatible Materials (administration & dosage, chemistry)
• Dose-Response Relationship, Immunologic
• Female
• Immunity, Cellular (immunology)
• Immunoglobulin G (blood)
• Mice
• Mice, Inbred BALB C
• Microspheres
• Nasal Mucosa (immunology)
• Plague (immunology, prevention & control)
• Polyesters (administration & dosage, chemistry)
• Pore Forming Cytotoxic Proteins
• Vaccines, Synthetic (administration & dosage, immunology)
• Yersinia pestis (genetics, immunology)