Abstract |
The polyubiquitin gene of the human protozoan parasite Plasmodium falciparum (PfpUB) was cloned and shown to be comprised of five tandem repeats of the ubiquitin open reading frame, present as a single copy on chromosome 12. The 1672 bp of PfpUB is interrupted at the 5' end by a single intron of 526 bp. PfpUB expression is developmentally regulated in intraerythrocytic stages with a marked increase in both steady-state transcript and polyubiquitin protein levels in late trophozoite stages. On response to heat shock, late stage parasites (late trophozoites and schizonts) have a slightly elevated PfpUB transcript level as well as readily observable increases in the amount of polyubiquitin and ubiquitin-conjugated proteins.
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Authors | P Horrocks, C I Newbold |
Journal | Molecular and biochemical parasitology
(Mol Biochem Parasitol)
Vol. 105
Issue 1
Pg. 115-25
(Jan 05 2000)
ISSN: 0166-6851 [Print] Netherlands |
PMID | 10613704
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biopolymers
- DNA, Protozoan
- Ubiquitins
- Polyubiquitin
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Topics |
- Amino Acid Sequence
- Animals
- Biopolymers
(chemistry, genetics, metabolism)
- Blotting, Northern
- Cloning, Molecular
- DNA, Protozoan
- Erythrocytes
(parasitology)
- Evolution, Molecular
- Gene Expression Regulation
- Heat-Shock Response
- Humans
- Molecular Sequence Data
- Open Reading Frames
- Plasmodium falciparum
(genetics, growth & development, metabolism)
- Polyubiquitin
- Sequence Analysis, DNA
- Tandem Repeat Sequences
(genetics)
- Transcription, Genetic
- Ubiquitins
(chemistry, genetics, metabolism)
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