Abstract |
Whereas ligation of the DNAM-1 adhesion molecule triggers cytotoxicity mediated by normal NK and T cells, this function was defective in NK cell clones from leukocyte adhesion deficiency syndrome. However, genetic reconstitution of cell surface expression of LFA-1 restored the ability of DNAM-1 to initiate anti-DNAM-1 mAb-induced cytotoxicity, indicating a functional relationship between DNAM-1 and LFA-1. Further studies demonstrated that LFA-1 physically associates with DNAM-1 in NK cells and anti-CD3 mAb stimulated T cells, for which serine phosphorylation of DNAM-1 plays a critical role. In addition, cross-linking of LFA-1 induces tyrosine phosphorylation of DNAM-1, for which the Fyn protein tyrosine kinase is responsible. These results indicate that DNAM-1 is involved in the LFA-1-mediated intracellular signals.
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Authors | K Shibuya, L L Lanier, J H Phillips, H D Ochs, K Shimizu, E Nakayama, H Nakauchi, A Shibuya |
Journal | Immunity
(Immunity)
Vol. 11
Issue 5
Pg. 615-23
(Nov 1999)
ISSN: 1074-7613 [Print] United States |
PMID | 10591186
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, Differentiation, T-Lymphocyte
- CD226 antigen
- CD3 Complex
- Cell Adhesion Molecules
- Lymphocyte Function-Associated Antigen-1
- Proto-Oncogene Proteins
- Phosphoserine
- FYN protein, human
- Fyn protein, mouse
- Proto-Oncogene Proteins c-fyn
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Topics |
- Animals
- Antibodies, Monoclonal
(immunology)
- Antigens, Differentiation, T-Lymphocyte
- CD3 Complex
(immunology)
- COS Cells
- Cell Adhesion
- Cell Adhesion Molecules
(chemistry, immunology, physiology)
- Chlorocebus aethiops
- Cytotoxicity, Immunologic
- Genetic Complementation Test
- Humans
- Jurkat Cells
- Killer Cells, Natural
(immunology)
- Leukocyte-Adhesion Deficiency Syndrome
(immunology, pathology)
- Lymphocyte Activation
- Lymphocyte Function-Associated Antigen-1
(chemistry, physiology)
- Mice
- Phosphorylation
- Phosphoserine
(metabolism)
- Protein Processing, Post-Translational
- Proto-Oncogene Proteins
(physiology)
- Proto-Oncogene Proteins c-fyn
- Signal Transduction
(physiology)
- T-Lymphocytes
(immunology)
- Tumor Cells, Cultured
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