Inhibitory activity of nm23-H1 on invasion and colonization of human prostate carcinoma cells is not mediated by its NDP kinase activity.

Abstract

The human nm23-H1 gene product, a putative metastasis suppressor, was identified as nucleoside diphosphate kinase (NDPK) A isoform. To investigate the functional effect of nm23-H1's NDPK activity on its suppression of the components of metastatic phenotype, we transfected a human prostate carcinoma cell line, DU145, with the cDNA encoding nm23-H1 mutant protein lacking NDPK activity. The mutant nm23-H1 transfected cell lines displayed decreased invasiveness and colonization in soft agar as the wild-type nm23-H1 transfectants did when compared with the control transfected line. The results suggest that the metastasis suppressing function of nm23-H1 is independent of the NDPK enzymatic activity.

• Authors: H Y Lee, H Lee
• Journal: Cancer letters (Cancer Lett) Vol. 145 Issue 1-2 Pg. 93-9 (Oct 18 1999) ISSN: 0304-3835 [Print] Ireland
• PMID: 10530775 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• NM23 Nucleoside Diphosphate Kinases
• Transcription Factors
• NME1 protein, human
• Nucleoside-Diphosphate Kinase
• Monomeric GTP-Binding Proteins

Topics

• Carcinoma (drug therapy, pathology, secondary)
• Humans
• Male
• Monomeric GTP-Binding Proteins
• Mutation
• NM23 Nucleoside Diphosphate Kinases
• Neoplasm Invasiveness
• Nucleoside-Diphosphate Kinase (metabolism)
• Phenotype
• Prostatic Neoplasms (drug therapy, enzymology, pathology)
• Transcription Factors (genetics, therapeutic use)
• Transfection
• Tumor Cells, Cultured
• Tumor Stem Cell Assay