Ischemia-reperfusion injury, a common source of renal dysfunction in adults, is associated with tubular epithelial cell damage. Since
fibroblast growth factors (FGF) attenuated tissue injury after transient
myocardial ischemia, we hypothesized that
acidic fibroblast growth factor (aFGF; FGF-1) would attenuate renal
ischemia-reperfusion injury. We studied the effects of
FGF-1 in a rat model of
acute renal failure induced by bilateral renal
ischemia (60 min) and 1, 2 or 7 days reperfusion. After
FGF-1 administration at the onset of renal reperfusion, there was less functional impairment of the kidneys. The histological changes were not as severe as in controls. Increases in serum
creatinine and blood
urea nitrogen 24 h after reperfusion were attenuated by 35% (p< 0.01) and by 53% (p< 0.001), respectively, in FGF-1-treated animals compared to vehicle-treated rats. The
ischemia/reperfusion-induced increase in tissue
myeloperoxidase, a marker of neutrophil infiltration, was mitigated (67% reduction, p< 0.05) with
FGF-1 treatment. As shown by histology, neutrophil infiltration and tubular cell
necrosis in medulla were less pronounced (p< 0.0001 and p< 0.05, respectively) in animals receiving
FGF-1. Furthermore,
ischemia-induced apoptosis, prevalent in tubular cells of the cortex, was also attenuated by FGF-1-treatment (83% reduction, p< 0.0001). Pretreatment of animals with Nw-nitro-
L-arginine (L-NNA), an inhibitor of
nitric oxide synthase, abolished the attenuating effects of
FGF-1 on neutrophil infiltration, suggesting that
nitric oxide might participate in the anti-inflammatory effects of
FGF-1 in this experimental design. Our data support a role for
FGF-1 in attenuation of renal damage or failure after
ischemia-reperfusion injury of the kidney, in part at least by inhibition of neutrophil infiltration.