Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum.

Abstract

The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.

• Authors: P Uparanukraw, T Harnnoi, A Dantrakool, N Morakote, W Chaicumpa
• Journal: Journal of helminthology (J Helminthol) Vol. 73 Issue 3 Pg. 273-6 (Sep 1999) ISSN: 0022-149X [Print] England
• PMID: 10526422 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, Helminth
• Helminth Proteins

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antigens, Helminth (analysis)
• Eels (parasitology)
• Glycosylation
• Gnathostoma (immunology)
• Helminth Proteins (analysis, immunology)
• Humans
• Larva (immunology)
• Radioimmunoprecipitation Assay
• Spirurida Infections (immunology)