Potential role of glycerol leading to rat fructose hypertension.

Abstract

A fructose-enriched diet promotes hypertension in rats. We thought that an enhancement of the glycolytic and/or lipid disorder (s) that raise blood pressure could be the cause. Therefore, we studied 4 groups of Sprague-Dawley rats (+/-200 g): (1) control rats received a standard diet and tap water; (2) the glycerol group of rats received a standard diet and 0.54 mol/L glycerol in tap water; (3) the fructose group was given a fructose-enhanced diet (chow had 55% fructose instead of dextrin) and tap water; and (4) the fructose-glycerol group was given the fructose-enhanced diet and 0. 54 mol/L glycerol in drinking water. At the end of the second week, the findings were as follows. Blood pressure was 149+/-2 mm Hg in the fructose-glycerol group versus 129+/-2 (P<0.001), 131+/-2 (P<0. 001), and 140+/-3 (P<0.005) mm Hg in the control, glycerol, and fructose groups, respectively. Insulinemia was higher in the fructose-glycerol group than the control (P<0.001), glycerol (P<0. 001), and fructose groups (P<0.001); triglyceridemia was higher in the fructose-glycerol (P<0.02), fructose (P<0.05), and glycerol groups (P<0.02) than the control group. Thoracic aorta rings showed a lower ED(50) to 12,13-phorbol dibutyrate in the fructose-glycerol group than in the control (P<0.001), glycerol (P<0.002), and fructose groups (P<0.001). In conclusion, glycerol-fructose administration resulted in hypertriglyceridemia, hyperinsulinemia, and increased vascular sensitivity to 12,13-phorbol dibutyrate (with respect to the control group), and significantly greater expression of protein kinase C alpha and betaII (with respect to the glycerol group).

• Authors: P F Damiano, M I Rosón, I Armando, S Nowicki, E Dascal, L Cuniberti, L E Albornoz, I J de la Riva
• Journal: Hypertension (Dallas, Tex. : 1979) (Hypertension) Vol. 34 Issue 4 Pt 2 Pg. 1007-11 (Oct 1999) ISSN: 0194-911X [Print] United States
• PMID: 10523399 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Insulin
• Fructose
• Glycerol

Topics

• Animals
• Blood Pressure (drug effects)
• Diet
• Drug Synergism
• Fructose (administration & dosage)
• Glycerol (pharmacology)
• Hypertension (chemically induced, metabolism, physiopathology)
• Insulin (metabolism)
• Male
• Rats
• Rats, Sprague-Dawley