Abstract | BACKGROUND: METHODS: Eighty women (19 to 74 years of age) were included in this study and grouped according to the Bethesda System: within normal limits (WNL), LSIL, and HSIL. By FISH, chromosomes 7 and X, and in part chromosome 3, were analyzed for numerical aberrations. Using the hybrid capture system HPV detection was performed. RESULTS: All 20 patients with cervical smear WNL had regular diploid chromosomal pattern and were negative for HPV. Thirteen of the 29 (41.2%) patients with LSIL showed trisomy 7, in association with trisomy X in 4 cases (12.9%). Single trisomy X was detected in 4 cases (12.9%). In 3 of 15 (20%) cases analyzed for chromosome 3 trisomy was observed. Trisomy 3 was associated with trisomy 7 and X or with trisomy 7 alone. The hybrid capture test was performed in 16 patients of this group. Two patients were positive for HPV probe A, 9 for probe B, and 2 for A and B, and 3 patients were negative. Twenty-three of the 29 patients (79.3%) with HSIL showed trisomy 7. Twelve of the 29 patients (41.3%) had an additional trisomy X. Single trisomy X was seen in only 2 cases (6. 9%). Twenty-two patients with HSIL were tested also for chromosome 3. Nine of the 22 patients (40.9%) showed trisomy 3, associated with trisomy 7 or with trisomy 7 and X. In 25 of the 29 patients HPV detection by the hybrid capture system was performed. HPV probe B was positive in 15 cases (60%). One patient was positive for both probes, A and B. Nine (36%) of the patients with HSIL were negative for both HPV probes. No positivity was observed for HPV probe A alone. CONCLUSION: Our data confirm the pivotal role of HPV in cervical carcinogenesis as it seems to cause changes in the chromosomal pattern of premalignant lesions. Additionally, trisomy 7 may be considered an early event in cervical carcinogenesis, persisting and increasing with progression of the lesion. The roles of trisomy 3 and X need further evaluation.
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Authors | C Mian, D Bancher, P Kohlberger, C Kainz, A Haitel, K Czerwenka, J Stani, G Breitenecker, H Wiener |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 75
Issue 1
Pg. 41-6
(Oct 1999)
ISSN: 0090-8258 [Print] United States |
PMID | 10502423
(Publication Type: Journal Article)
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Copyright | Copyright 1999 Academic Press. |
Topics |
- Adult
- Aged
- Chromosome Aberrations
(genetics)
- Chromosomes, Human, Pair 3
(genetics)
- Chromosomes, Human, Pair 7
(genetics)
- Female
- Humans
- In Situ Hybridization, Fluorescence
- Middle Aged
- Papillomaviridae
(isolation & purification)
- Papillomavirus Infections
(complications, virology)
- Tumor Virus Infections
(complications, virology)
- Uterine Cervical Neoplasms
(complications, genetics, pathology, virology)
- Vaginal Smears
- X Chromosome
(genetics)
- Uterine Cervical Dysplasia
(complications, genetics, pathology, virology)
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