Host cell protein kinases in nonsegmented negative-strand virus (mononegavirales) infection.

Abstract

Phosphorylation of one or more viral proteins is probably an essential step in the life cycle of every member of the nonsegmented negative-strand RNA virus (mononegavirales [MNV]) group. Since no virally encoded protein kinases have been discovered in this group, phosphorylation is effected entirely by host cell kinases. The virally encoded P proteins of the MNV are the only ones consistently phosphorylated with a stoichiometry > or =1. The P protein of vesicular stomatitis virus (VSV), and perhaps also of respiratory syncytial virus, are the only ones for which a function of phosphorylation has been established. Phosphorylation by casein kinase 2 at one or more identified sites in the VSV P protein activates transcriptional activity by promoting formation of a homotrimer, which is then capable of binding the RNA polymerase and attaching it to the N protein-RNA template. A second phosphorylation of VSV P protein by a different kinase also occurs, dependent upon prior modification by casein kinase 2, but its function is not definitely known. Phosphorylation of the other MNV P proteins may serve a different purpose. No evidence has been obtained yet for any function for phosphorylation of any other MNV protein.

• Authors: J Lenard
• Journal: Pharmacology & therapeutics (Pharmacol Ther) Vol. 83 Issue 1 Pg. 39-48 (Jul 1999) ISSN: 0163-7258 [Print] England
• PMID: 10501594 (Publication Type: Journal Article, Review)

Chemical References

• Protein Kinases
• Casein Kinases

Topics

• Casein Kinases
• Humans
• Mononegavirales (enzymology, metabolism)
• Mononegavirales Infections (enzymology)
• Phosphorylation
• Protein Kinases (metabolism, physiology)
• Transcription, Genetic
• Vesicular stomatitis Indiana virus (enzymology, metabolism)