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Mast cells cause apoptosis of cardiomyocytes and proliferation of other intramyocardial cells in vitro.

AbstractBACKGROUND:
Mast cells are multifunctional cells containing various mediators such as cytokines, proteases, and histamine. They are found in the human heart and have been implicated in ventricular hypertrophy and heart failure. However, their roles in pathogenesis of these diseases are unknown.
METHODS AND RESULTS:
Cultured cardiomyocytes from neonatal rats were incubated with mast cell granules (MCGs) for 24 hours. The highest concentration of diluted MCGs caused the death of approximately 70% of cardiomyocytes. This cell death was proved to be apoptosis, as quantified by electron microscopy and biochemical criteria. MCG-mediated cytotoxicity was prevented by pretreatment of MCGs with protease inhibitors or a neutralizing antibody against rat mast cell chymase 1 (RMCP 1). RMCP 1 by itself was proved to induce cell death of cardiomyocytes. These results suggest that RMCP 1 contained in MCGs causes the death of cardiomyocytes. In contrast, MCGs induced the proliferation of intramyocardial cells other than myocytes. RMCP 1 was also proved to induce their proliferation.
CONCLUSIONS:
Mast cells cause apoptosis of cardiomyocytes and proliferation of other intramyocardial cells via the activity of RMCP 1. Our results suggest that mast cell chymase may play a role in the progression of heart failure, because loss of cardiomyocytes and proliferation of nonmyocardial cells exaggerate its pathophysiology.
AuthorsM Hara, A Matsumori, K Ono, H Kido, M W Hwang, T Miyamoto, A Iwasaki, M Okada, K Nakatani, S Sasayama
JournalCirculation (Circulation) Vol. 100 Issue 13 Pg. 1443-9 (Sep 28 1999) ISSN: 1524-4539 [Electronic] United States
PMID10500047 (Publication Type: Journal Article)
Chemical References
  • Histamine
  • Endopeptidases
  • Serine Endopeptidases
  • CMA1 protein, human
  • Chymases
  • Cma1 protein, rat
Topics
  • Animals
  • Apoptosis (physiology)
  • Cell Death (drug effects, physiology)
  • Cell Division (drug effects, physiology)
  • Cells, Cultured
  • Chymases
  • Cytoplasmic Granules (physiology)
  • Endopeptidases (physiology)
  • Histamine (pharmacology)
  • Male
  • Mast Cells (enzymology, physiology)
  • Myocardium (cytology)
  • Rats
  • Rats, Sprague-Dawley
  • Serine Endopeptidases (pharmacology)

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