C-C chemokines in allergen-induced late-phase cutaneous responses in atopic subjects: association of eotaxin with early 6-hour eosinophils, and of eotaxin-2 and monocyte chemoattractant protein-4 with the later 24-hour tissue eosinophilia, and relationship to basophils and other C-C chemokines (monocyte chemoattractant protein-3 and RANTES).

Abstract

The relationship of expression of the C-C chemokines eotaxin, eotaxin 2, RANTES, monocyte chemoattractant protein-3 (MCP-3), and MCP-4 to the kinetics of infiltrating eosinophils, basophils, and other inflammatory cells was examined in allergen-induced, late-phase allergic reactions in the skin of human atopic subjects. EG2+ eosinophils peaked at 6 h and correlated significantly with eotaxin mRNA and protein, whereas declining eosinophils at 24 h correlated significantly with eotaxin-2 and MCP-4 mRNA. In contrast, no significant correlations were observed between BB1+ basophil infiltrates, which peaked at 24 h, and expression of eotaxin, eotaxin-2, RANTES, MCP-3, and MCP-4 or elastase+ neutrophils (6-h peak), CD3+ and CD4+ T cells (24 h), and CD68+ macrophages (72 h). Furthermore, 83% of eosinophils, 40% of basophils, and 1% of CD3+ cells expressed the eotaxin receptor CCR3, while eotaxin protein was expressed by 43% of macrophages, 81% of endothelial cells, and 6% of T cells (6%). These data suggest that 1) eotaxin has a role in the early 6-h recruitment of eosinophils, while eotaxin-2 and MCP-4 appear to be involved in later 24-h infiltration of these CCR3+ cells; 2) different mechanisms may guide the early vs late eosinophilia; and 3) other chemokines and receptors may be involved in basophil accumulation of allergic tissue reactions in human skin.

• Authors: S Ying, D S Robinson, Q Meng, L T Barata, A R McEuen, M G Buckley, A F Walls, P W Askenase, A B Kay
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 163 Issue 7 Pg. 3976-84 (Oct 01 1999) ISSN: 0022-1767 [Print] United States
• PMID: 10491000 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• CCL11 protein, human
• CCL13 protein, human
• CCL24 protein, human
• CCL7 protein, human
• CCR3 protein, human
• Chemokine CCL11
• Chemokine CCL24
• Chemokine CCL5
• Chemokine CCL7
• Chemokines, CC
• Cytokines
• Monocyte Chemoattractant Proteins
• RNA, Messenger
• Receptors, CCR3
• Receptors, Chemokine

Topics

• Adolescent
• Adult
• Allergens (immunology)
• Basophils (immunology, metabolism, pathology)
• Cell Movement (immunology)
• Chemokine CCL11
• Chemokine CCL24
• Chemokine CCL5 (genetics, metabolism)
• Chemokine CCL7
• Chemokines, CC (biosynthesis, genetics, metabolism)
• Cytokines (genetics, metabolism)
• Dermatitis, Atopic (immunology, pathology)
• Eosinophilia (immunology, pathology)
• Eosinophils (immunology, metabolism, pathology)
• Humans
• Immunophenotyping
• Middle Aged
• Monocyte Chemoattractant Proteins (genetics, metabolism)
• RNA, Messenger (biosynthesis)
• Receptors, CCR3
• Receptors, Chemokine (biosynthesis, genetics)
• Time Factors