Abstract | BACKGROUND/AIMS: METHODS: Genomic DNA extracted from 38 fresh samples of hepatocellular carcinoma was amplified by polymerase chain reaction using 29 fluorescence-labeled microsatellite markers and analyzed using a semi-automated laser scanning system. Associations between the incidence of replication error and the clinicopathological features of hepatocellular carcinoma were evaluated. Since reference DNA was extracted from corresponding fresh samples of non-cancerous liver tissue, the incidence of microsatellite instability in non-cancerous liver tissues was not assessed in this study. RESULTS: CONCLUSIONS: The results of this study indicate that: (1) microsatellite instability is a rare event during hepatocarcinogenesis and may be specifically associated with progression of hepatocellular carcinoma; and (2) frame-shift mutation in the above six genes is not a common mechanism involved in progression of this cancer.
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Authors | Y Kondo, Y Kanai, M Sakamoto, M Mizokami, R Ueda, S Hirohashi |
Journal | Journal of hepatology
(J Hepatol)
Vol. 31
Issue 3
Pg. 529-36
(Sep 1999)
ISSN: 0168-8278 [Print] Netherlands |
PMID | 10488714
(Publication Type: Clinical Trial, Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Aged
- Carcinoma, Hepatocellular
(genetics)
- Female
- Fluorescence
- Genotype
- Humans
- Lasers
- Liver Neoplasms
(genetics)
- Loss of Heterozygosity
- Male
- Microsatellite Repeats
- Middle Aged
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