[Preparation of recombinant polypeptides containing antigenic determinants of hepatitis E virus].

Abstract

DNA fragments complementary to hepatitis E Burma strain ORF2 and ORF3 obtained by oligonucleotide synthesis were cloned in expressing bacterial system. Recombinant polypeptides isolated from E. coli producer strains, immobilized on solid phase (polystyrene plates and nitrocellulose membranes), are studied in enzyme immunoassay to detect their ability to react with sera of patients with acute viral hepatitis from an Uzbekistan region endemic for hepatitis E. Two polypeptides reacting with the greatest number of sera, containing hepatitis E virus ORF2 and ORF3 gene fragments, were selected for further study of antigenic specificity.

• Authors: G I Alatortseva, A A Grinev, I I Amiantova, L N Nesterenko, L L Sukhanova, A V Titaev, V V Zverev, V A Gol'tsov
• Journal: Voprosy virusologii (Vopr Virusol) 1998 Nov-Dec Vol. 43 Issue 6 Pg. 266-9 ISSN: 0507-4088 [Print] Russia (Federation)
• Vernacular Title: Poluchenie rekombinantnykh polipeptidov, soderzhashchikh antigennye determinanty virusa gepatita E.
• PMID: 10488530 (Publication Type: Journal Article)

Chemical References

• DNA, Recombinant
• Epitopes
• Recombinant Proteins

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• DNA, Recombinant
• Epitopes (genetics, immunology)
• Escherichia coli (genetics)
• Hepatitis E virus (chemistry, immunology)
• Hepatitis, Viral, Human (virology)
• Humans
• Immunoenzyme Techniques
• Molecular Sequence Data
• Open Reading Frames
• Recombinant Proteins (genetics, immunology)