Protection against Staphylococcus aureus sepsis by vaccination with recombinant staphylococcal enterotoxin A devoid of superantigenicity.

Abstract

Staphylococcal exotoxins are virulence determinants in Staphylococcus aureus arthritis and septicemia. To assess the utility of enterotoxins as vaccine candidates for these diseases, a genetically modified staphylococcal enterotoxin A (SEA) that lacks superantigenic properties was used. Mice immunized with recombinant (r) SEA had significantly longer survival than control immunized mice and lost significantly less weight than the controls. Transfer of SEA-specific antibodies to naive mice resulted in good protection against death in staphylococcal sepsis. In vitro proliferative responses to SEA by naive lymphocytes were almost totally abolished on incubation with serum from rSEA but not with control antigen-immunized mice. These results suggest that immunization with rSEA devoid of superantigenic properties provides good protection against S. aureus sepsis. In addition, the data indicate that the protection is at least in part mediated by SEA neutralizing antibodies.

• Authors: I M Nilsson, M Verdrengh, R G Ulrich, S Bavari, A Tarkowski
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 180 Issue 4 Pg. 1370-3 (Oct 1999) ISSN: 0022-1899 [Print] United States
• PMID: 10479175 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Vaccines
• Enterotoxins
• Vaccines, Synthetic
• enterotoxin A, Staphylococcal

Topics

• Animals
• Bacteremia (immunology, prevention & control)
• Bacterial Vaccines
• Enterotoxins (immunology)
• Immunization, Passive
• Male
• Mice
• Mice, Inbred Strains
• Staphylococcal Infections (immunology, prevention & control)
• Staphylococcus aureus (immunology, pathogenicity)
• Vaccines, Synthetic (immunology)
• Virulence