Exogenous cytokine modulation or neutralization of interleukin-10 enhance survival in lipopolysaccharide-hyporesponsive C3H/HeJ mice with Klebsiella infection.

Abstract	Klebsiella pneumoniae has been isolated from liver abscesses in patients with leukaemia or diabetes. The resistance of Klebsiella infection in lipopolysaccharide (LPS)-hyporesponsive mice is unclear. Female C3H/HeJ and C3H/HeN mice, 6-8 weeks old, were intraperitoneally (i.p.) injected with K. pneumoniae. The results showed that C3H/HeJ mice were 24 times more susceptible [lethal dose 50% (LD50) 250 colony-forming units] than C3H/HeN mice to K. pneumoniae infection. C3H/HeJ mice, uninfected or infected with K. pneumoniae, had higher liver interleukin (IL)-10 levels and IL-10 mRNA levels than C3H/HeN mice. Previously, pretreatment with IL-1beta and tumour necrosis factor-alpha (TNF-alpha) protected C3H/HeJ mice from lethal bacterial infection. Therefore the effects of pretreatment with IL-1beta and TNF-alpha or antimurine IL-10 antibody i.p. 1 hr before this infection in both strains of C3H mice were examined. Pretreatment with TNF-alpha or anti-IL-10 antibody enhanced the survival of both strains of mice. TNF-alpha, in combination with IL-1beta, enhanced the survival and bacterial clearance better than single pretreatment in C3H/HeJ mice. Anti-IL-10 antibody increased bacterial clearance and significantly reduced liver cytokine mRNA levels in C3H/HeJ mice more than it did in the controls during infection. These results indicate that exogenous cytokine modulation or neutralization of IL-10 enhance the resistance of LD50 infection in C3H/HeJ mice.
Authors	M Wang, K C Jeng, L I Ping
Journal	Immunology (Immunology) Vol. 98 Issue 1 Pg. 90-7 (Sep 1999) ISSN: 0019-2805 [Print] England
PMID	10469238 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antibodies, Monoclonal Interleukin-1 Lipopolysaccharides RNA, Messenger Tumor Necrosis Factor-alpha Interleukin-10
Topics	Animals Antibodies, Monoclonal (therapeutic use) Colony Count, Microbial Female Immunotherapy Interleukin-1 (therapeutic use) Interleukin-10 (genetics, immunology) Klebsiella Infections (immunology, prevention & control) Klebsiella pneumoniae Lethal Dose 50 Lipopolysaccharides (immunology) Liver (immunology) Mice Mice, Inbred C3H RNA, Messenger (analysis) Statistics, Nonparametric Tumor Necrosis Factor-alpha (therapeutic use)

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