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SV40 small T antigen enhances progression to >G2 during lytic infection.

Abstract
The infection of monkey kidney (CV-1) cells with simian virus 40 (SV40) stimulates the cells into successive rounds of DNA synthesis without an intervening mitosis, leading to the acquisition of a >G2 DNA content. To elucidate the role of small t antigen in cell cycle progression and in viral replication during infection, studies were performed using an SV40 mutant (dl888) that lacks the ability to produce small t. Initially dl888-infected cells move through the first S phase at roughly the same rate as wild-type infected cells. Upon reaching G2, however, the dl888-infected cells progressed to >G2 at a reduced rate relative to wild-type. The slower rate of entry into >G2 of dl888-infected cells is associated with a decrease in total pRb and an increase in the ratio of hypophosphorylated to hyperphosphorylated pRb. The expression of cyclin D1 and p27(kip1) were elevated in dl888-infected cells compared to wild-type-infected CV-1 cells. Taken together, these results indicate that small t antigen plays a role in stimulating entry into >G2 in SV40-infected CV-1 cells, possibly by affecting the regulation of key cell cycle proteins.
AuthorsB Whalen, J Laffin, T D Friedrich, J M Lehman
JournalExperimental cell research (Exp Cell Res) Vol. 251 Issue 1 Pg. 121-7 (Aug 25 1999) ISSN: 0014-4827 [Print] United States
PMID10438577 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
CopyrightCopyright 1999 Academic Press.
Chemical References
  • Antigens, Polyomavirus Transforming
  • Cell Cycle Proteins
  • Microtubule-Associated Proteins
  • Retinoblastoma Protein
  • Tumor Suppressor Proteins
  • Cyclin D1
  • Cyclin-Dependent Kinase Inhibitor p27
  • Mimosine
  • DNA
Topics
  • Animals
  • Antigens, Polyomavirus Transforming (genetics, physiology)
  • Blotting, Western
  • Cell Cycle (drug effects)
  • Cell Cycle Proteins
  • Cell Line
  • Cell Nucleus (genetics)
  • Chlorocebus aethiops
  • Cyclin D1 (analysis)
  • Cyclin-Dependent Kinase Inhibitor p27
  • DNA (biosynthesis)
  • G2 Phase
  • Gene Expression Regulation
  • Kinetics
  • Microtubule-Associated Proteins (analysis)
  • Mimosine (pharmacology)
  • Mutation
  • Phosphorylation
  • Polyploidy
  • Retinoblastoma Protein (analysis, metabolism)
  • Simian virus 40 (genetics, physiology)
  • Tumor Suppressor Proteins
  • Virus Replication

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