Quantification of lamivudine (3TC) by competitive immunoassay.

Abstract

Lamivudine or 3TC, the (-) eniantiomer of 2'-deoxy-3'-thiacytidine, is a prototype of a novel class of levogyre dideoxynucleosides analogues used in treatment of HIV and HBV infection. We describe a method corresponding to the first enzyme immunoassay for quantifying this antiviral drug. This technique use an enzyme conjugate that not require the use of radioactive labelling. In this study, anti-3TC antibodies were raised in rabbits by immunising with 3TC-HS-kelhoyle limpet hemocyanin (KLH) conjugate.

• Authors: F Akeb, C Creminon, M C Nevers, J Grassi, D Duval, R Guedj
• Journal: Nucleosides & nucleotides (Nucleosides Nucleotides) 1999 Apr-May Vol. 18 Issue 4-5 Pg. 893-6 ISSN: 0732-8311 [Print] United States
• PMID: 10432705 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-HIV Agents
• Reverse Transcriptase Inhibitors
• Lamivudine

Topics

• Anti-HIV Agents (analysis, immunology)
• Cross Reactions
• Immunoassay (methods)
• Lamivudine (analysis, immunology)
• Reverse Transcriptase Inhibitors (analysis, immunology)