[Cloning of the idiopathic azoospermia related genes with mRNA differential display method].

Abstract

OBJECTIVE:

Defect of some genes related to spermatogenesis may result in male infertility with azoospermia. This study aimed to clone some of the genes.

METHODS:

Testis tissues from a patient with idiopathic azoospermia and from a normal fathered man were investigated with an improved mRNA differential display approach.

RESULTS:

Significant difference was observed between the two tissues in gene expression. Five differential expressed sequence-tags (ESTs) were cloned and sequenced. Homology analysis with software advanced BLAST 2.0 showed that one EST shared 100% homology with cosmid L27h9, which located in Huntington's disease region on 4p16.3; the other 4 showed very low homology with sequences in GenBank.

CONCLUSION:

Azoospermia has complex genetic heterogeneity. A gene located in Huntington's disease region on 4p16.3 is expressed in human testis during the spermatogenesis, and a loss of its function may associate with azoospermia.

• Authors: J Yang, C Xiao, S Zhang, M Huang, T Fan, Q Xia
• Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (Zhonghua Yi Xue Yi Chuan Xue Za Zhi) Vol. 16 Issue 4 Pg. 220-3 (Aug 1999) ISSN: 1003-9406 [Print] China
• PMID: 10431046 (Publication Type: English Abstract, Journal Article)

Chemical References

• RNA, Messenger

Topics

• Cloning, Organism
• Humans
• Huntington Disease (genetics)
• Male
• Oligospermia (genetics)
• RNA, Messenger (analysis)
• Sequence Homology, Amino Acid