A polyketide synthase gene required for biosynthesis of fumonisin mycotoxins in Gibberella fujikuroi mating population A.

Abstract	Fumonisins are toxins associated with several mycotoxicoses and are produced by the maize pathogen Gibberella fujikuroi mating population A (MP-A). Biochemical analyses indicate that fumonisins are a product of either polyketide or fatty acid biosynthesis. To isolate a putative polyketide synthase (PKS) gene involved in fumonisin biosynthesis, we employed PCR with degenerate PKS primers and a cDNA template prepared from a fumonisin-producing culture of G. fujikuroi. Sequence analysis of the single PCR product and its flanking DNA revealed a gene (FUM5) with a 7.8-kb coding region. The predicted FUM5 translation product was highly similar to bacterial and fungal Type I PKSs. Transformation of a cosmid clone carrying FUM5 into G. fujikuroi enhanced production in three strains and restored wild-type production in a fumonisin nonproducing mutant. Disruption of FUM5 reduced fumonisin production by over 99% in G. fujikuroi MP-A. Together, these results indicate that FUM5 is a PKS gene required for fumonisin biosynthesis.
Authors	R H Proctor, A E Desjardins, R D Plattner, T M Hohn
Journal	Fungal genetics and biology : FG & B (Fungal Genet Biol) Vol. 27 Issue 1 Pg. 100-12 (Jun 1999) ISSN: 1087-1845 [Print] United States
PMID	10413619 (Publication Type: Journal Article)
Copyright	Copyright 1999 Academic Press.
Chemical References	Carboxylic Acids DNA Primers DNA, Fungal Fumonisins Isoenzymes Multienzyme Complexes Mycotoxins fumonisin B2 fumonisin B3 fumonisin B1 beta-ketoacyl-acyl carrier protein synthase I 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase
Topics	3-Oxoacyl-(Acyl-Carrier-Protein) Synthase (genetics) Amino Acid Sequence Carboxylic Acids (metabolism) DNA Primers DNA, Fungal (genetics) Fumonisins Genes, Fungal (genetics) Genetic Complementation Test Genetic Vectors Gibberella (enzymology, genetics) Isoenzymes (genetics) Molecular Sequence Data Multienzyme Complexes (genetics, metabolism) Mutagenesis, Site-Directed Mutation Mycotoxins (biosynthesis) Polymerase Chain Reaction Sequence Homology, Amino Acid Transformation, Genetic

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