[The classical and alternative pathways of T-lymphocyte activation in patients with systemic lupus erythematosus].

Abstract

AIM:

To study activation of T-lymphocytes by the CD3 (antigen-dependent) and CD2 (non-antigen-dependent) routes in patients with systemic lupus erythematosus (SLE).

MATERIALS AND METHODS:

Peripheral blood mononuclears were studied in 66 patients with SLE and 27 donors. Proliferative response to activation by anti-CD3, anti-CD3+ phorbol-12-myristate-13-acetate (PMA), phytohemagglutinin (PHA), and autologous erythrocytes in combinations with PMA and recombinant interleukin-2 (rIL-2) was assessed.

RESULTS:

T-cell proliferation was at least two times increased under the effect of CD3 in 40.9% patients and in 100% normal subjects. Stimulation with CD3 antibodies in combination with PMA leveled the differences due to boosting of T-cell response in SLE patients. PMA alone caused mononuclear proliferation in 25% patients with SLE but not in normal subjects. Decreased response of T-cells to adhesive stimulus (autologous erythrocytes + PMA) in SLE patients was leveled by rIL-2.

CONCLUSION:

The proliferative response of T-lymphocytes is decreased upon stimulation with CD3 and CD2 and in some patients increased by PMA in submitogenic doses, added alone or in combination with anti-CD3.

• Authors: M L Stanislav, R M Balabanova, M F Nikonova, M M Litvina, A A Iarilin
• Journal: Terapevticheskii arkhiv (Ter Arkh) Vol. 71 Issue 5 Pg. 30-4 ( 1999) ISSN: 0040-3660 [Print] Russia (Federation)
• Vernacular Title: Sostoianie klassicheskogo i al'ternativnogo puteĭ aktivatsii T-limfotsitov u bol'nykh sistemnoĭ krasnoĭ volchanki.
• PMID: 10399227 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, Surface
• CD2 Antigens
• CD3 Complex

Topics

• Adult
• Antibodies, Monoclonal (immunology)
• Antigens, Surface (immunology)
• Autoimmune Diseases (immunology)
• CD2 Antigens (immunology)
• CD3 Complex (immunology)
• Female
• Flow Cytometry
• Humans
• Lupus Erythematosus, Systemic (immunology)
• Lymphocyte Activation (immunology)
• Male
• Middle Aged
• T-Lymphocytes (immunology)