Cross-reactivity of anti-HIV-1-p17-derivative peptide (P30-52) antibody to Env V3 peptide.

Abstract

Strong antibody responses are often seen in human immunodeficiency virus type 1 (HIV-1) carriers, but it is not known whether these antibodies are effective in the inhibition of disease progression. In this study, we examined antigenic epitopes for anti-HIV-1 p17 antibody (p17 Ab) in an HIV-1 carrier's serum, and found that the residues of amino acid numbers 1 to 12 (P1-12), 12 to 29 (P12-29) and 30 to 52 (P30-52) of p17 were highly recognized in the serum. Our examination of purified antibodies from the patient using the p17-derivative-peptide-immunoaffinity columns showed that the reactivity of anti-p30-52 Ab (p30-52Ab) was high for p30-52 and the naive protein, p17. In addition, this P30-52Ab cross-reacted with the third variable region of the envelope glycoprotein (Env V3). To confirm this cross-reactivity, we immunized mice with P30-52, and established a monoclonal antibody (MAb), 8H10. We found that 8H10 was also reactive to Env V3. It is unclear whether this cross-reactivity of P30-52 Ab can function as the inhibitor of HIV-1, but these results will be of help in clarifying the interaction of Env protein with HIV-1 gag polyprotein and the relationship of the decline of the p17 antibody titer with the disease progression in HIV-1 carriers.

• Authors: A Ota, K Tanaka-Taya, S Ueda
• Journal: Hybridoma (Hybridoma) Vol. 18 Issue 2 Pg. 149-57 (Apr 1999) ISSN: 0272-457X [Print] United States
• PMID: 10380014 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Epitopes
• Gene Products, gag
• HIV Antibodies
• HIV Antigens
• HIV Envelope Protein gp120
• HIV envelope protein gp120 (305-321)
• Peptide Fragments
• Recombinant Proteins
• Viral Proteins
• gag Gene Products, Human Immunodeficiency Virus
• p17 protein, Human Immunodeficiency Virus Type 1

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Monoclonal (immunology)
• Antibody Affinity
• Antibody Specificity
• Biosensing Techniques
• Carrier State
• Cross Reactions
• Enzyme-Linked Immunosorbent Assay
• Epitopes
• Female
• Gene Products, gag (immunology)
• HIV Antibodies (immunology)
• HIV Antigens (immunology)
• HIV Envelope Protein gp120 (immunology)
• HIV Infections (immunology)
• HIV-1 (immunology)
• Humans
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Peptide Fragments (immunology)
• Recombinant Proteins (immunology)
• Viral Proteins
• gag Gene Products, Human Immunodeficiency Virus