Saccharomyces boulardii upgrades cellular adaptation after proximal enterectomy in rats.

Abstract

BACKGROUND:

Saccharomyces boulardii is a non-pathogenic yeast which exerts trophic effects on human and rat small intestinal mucosa.

AIMS:

To examine the effects of S boulardii on ileal adaptation after proximal enterectomy in rats.

METHODS:

Wistar rats, aged eight weeks, underwent 60% proximal resection or transection and received by orogastric intubation either 1 mg/g body wt per day lyophilised S boulardii or the vehicle for seven days. The effects on ileal mucosal adaptation were assessed eight days after surgery.

RESULTS:

Compared with transection, resection resulted in mucosal hyperplasia with significant decreases in the specific and total activities of sucrase, lactase, and maltase. Treatment of resected animals with S boulardii had no effect on mucosal hyperplasia but did upgrade disaccharidase activities to the levels of the transected group. Enzyme stimulation by S boulardii was associated with significant increases in diamine oxidase activity and mucosal polyamine concentrations. Likewise, sodium dependent D-glucose uptake by brush border membrane vesicles, measured as a function of time and glucose concentration in the incubation medium, was significantly (p<0.05) increased by 81% and three times respectively in the resected group treated with S boulardii. In agreement with this, expression of the sodium/glucose cotransporter-1 in brush border membranes of resected rats treated with S boulardii was enhanced twofold compared with resected controls.

CONCLUSION:

Oral administration of S boulardii soon after proximal enterectomy improves functional adaptation of the remnant ileum.

• Authors: J P Buts, N De Keyser, S Marandi, D Hermans, E M Sokal, Y H Chae, L Lambotte, H Chanteux, P M Tulkens
• Journal: Gut (Gut) Vol. 45 Issue 1 Pg. 89-96 (Jul 1999) ISSN: 0017-5749 [Print] England
• PMID: 10369710 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Polyamines
• Disaccharidases
• Sodium-Potassium-Exchanging ATPase

Topics

• Adaptation, Physiological
• Animals
• Disaccharidases (metabolism)
• Ileum (enzymology, microbiology, physiopathology, surgery)
• Male
• Polyamines (metabolism)
• Postoperative Care (methods)
• Rats
• Rats, Wistar
• Saccharomyces
• Sodium-Potassium-Exchanging ATPase (metabolism)