Detailed transcript map of a 810-kb region at 11p14 involving identification of 10 novel human 3' exons.

A limited number of genes, including the human brain-derived neutrotrophic factor (BDNF) gene, have been identified in the human chromosome 11p14 region. Since this area is involved in a genetic disorder (WAGR syndrome) and because of interest in studying the regulation of the human BDNF gene, we have established a detailed transcript map of a 810-kb region clone in a yeast artificial chromosome (YAC), corresponding to a portion of this genomic locus. A set of nested deletion mutants has been generated to map genes at a mean resolution of 75kb. Four genic markers from available mapping databases have been mapped on the YAC. Ten potential novel human exons have been isolated by a 3' terminal exon trapping procedure directly applied to purified YAC DNA. Most of these exons display polyadenylation signals and they all yield positive signals in RT-PCR experiments, confirming their status of transcribed sequences. The BDNF gene is now co-localised with three other genes on a 120 kb DNA fragment.
AuthorsF Guillemot, C Auffray, M D Devignes
JournalEuropean journal of human genetics : EJHG (Eur J Hum Genet) 1999 May-Jun Vol. 7 Issue 4 Pg. 487-95 ISSN: 1018-4813 [Print] ENGLAND
PMID10352939 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Brain-Derived Neurotrophic Factor
  • Brain-Derived Neurotrophic Factor (genetics)
  • Chromosome Mapping
  • Chromosomes, Artificial, Yeast
  • Chromosomes, Human, Pair 11 (genetics)
  • Electrophoresis, Gel, Pulsed-Field
  • Exons (genetics)
  • Humans
  • Microsatellite Repeats (genetics)
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Transcription, Genetic
  • WAGR Syndrome (genetics)

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