Studies on the creatine kinase MM isoforms of normal and Duchenne muscular dystrophic patients.

Abstract

OBJECTIVE:

To study the changes of creatine kinase MM (CK-MM) isoforms in Duchenne muscular dystrophy (DMD) patients.

METHODS:

Serum samples from 49 DMD patients and 40 control subjects were collected for CK-MM isoforms measurement. CK-MM isoforms were separated within 30 minutes by electrophoresis on agarose gel with a discontinuous buffer system at constant current of 30 mA and low voltage of 200-300 V, then measured by fluorescence scanning.

RESULTS:

Significant differences of MM2/MM1 ratio were found between DMD patients and control subjects (P < 0.05) as well as among the three different age groups of DMD patients (P < 0.05).

CONCLUSIONS:

CK-MM isoforms may present useful information for the early diagnosis and evaluation of DMD and the ratio of MM2/MM1 can be considered as a specific indicator of the degree of seriousness for DMD patients.

• Authors: X Zhao, C Bi, Z Yang
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 111 Issue 1 Pg. 75-7 (Jan 1998) ISSN: 0366-6999 [Print] China
• PMID: 10322660 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Isoenzymes
• Creatine Kinase

Topics

• Adolescent
• Adult
• Biomarkers
• Child
• Child, Preschool
• Creatine Kinase (blood)
• Electrophoresis, Agar Gel
• Female
• Humans
• Isoenzymes
• Male
• Muscular Dystrophies (enzymology)