Abstract | BACKGROUND: MATERIALS AND METHODS: PC3 cells were exposed to Ad-C-CAM1 and the time course of C-CAM1 expression was monitored by flow cytometry. Tumors generated in nude mice by subcutaneous injection of PC-3 cells were used for in vivo testing of C-CAM1's antitumor activity. Intratumoral injections of viruses (either Ad-C-CAM1 or Ad-beta-gal) or buffer only (control) were performed according to two different schedules. Mice in Schedule A received a single injection, while mice in Schedule B received the same total amount of viruses in 3 equal doses at 2-week intervals. RESULTS: After single exposure to Ad-C-CAM1, PC-3 cells expressed abundant C-CAM1 protein which reached the highest level on day 3 and persisted for up to 5 days. PC-3 tumors in nude mice exhibited 2 to 3-week lag in tumor growth curves after a single Ad-C-CAM1 injection. In contrast, 14 of the 18 tumors receiving 3 fractionated Ad-C-CAM1 injections regressed completely, while the other 4 tumors shrank to significantly smaller sizes. CONCLUSIONS: Sustained expression of C-CAM1 is required for optimal tumor suppression. The schedule-dependence of C-CAM1's antitumor activity should be taken into account in optimizing gene therapy in clinical settings.
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Authors | S H Lin, Y S Pu, W Luo, Y Wang, C J Logothetis |
Journal | Anticancer research
(Anticancer Res)
1999 Jan-Feb
Vol. 19
Issue 1A
Pg. 337-40
ISSN: 0250-7005 [Print] Greece |
PMID | 10226564
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD
- CD66 antigens
- Carcinoembryonic Antigen
- Ceacam1 protein, mouse
- Ceacam2 protein, mouse
- Cell Adhesion Molecules
- Glycoproteins
- Adenosine Triphosphatases
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Topics |
- Adenosine Triphosphatases
(biosynthesis, genetics)
- Adenoviridae
(genetics)
- Animals
- Antigens, CD
- Carcinoembryonic Antigen
- Cell Adhesion Molecules
(biosynthesis, genetics)
- Genetic Therapy
- Glycoproteins
- Humans
- Male
- Mice
- Mice, Nude
- Prostatic Neoplasms
(therapy)
- Tumor Cells, Cultured
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