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Analysis of N-acetyl and N-glycolylneuraminic acid in rat serum and tissues with Walker 256 carcinoma by high-performance liquid chromatography.

Abstract
Serum and tissue specimens from healthy Wistar rats and from rats with Walker 256 carcinoma were analysed for N-acetyl and N-glycolylneuraminic acid by high performance liquid chromatography (HPLC) as per-O-benzoylated derivatives. Both neuraminic acids were identified, while N-acetylneuraminic acid was the predominant sialic acid. Samples from rats with generalized metastasis showed a significant increase (45-80%) of total sialic acids. This phenomenon in serum is caused by the overproduction of sialic acids, as a result of synthesis of both types of neuraminic acids to a similar molar ratio. The increase of sialic acids in rat bones with metastatic cancer is mainly because of increased N-acetylneuraminic acid synthesis. These results suggest that the molecular mechanisms responsible for cancer metastasis in different tissues may be closely associated with increased synthesis of dominating neuraminic acid.
AuthorsE Makatsori, A Aletras, N K Karamanos, T Tsegenidis
JournalBiomedical chromatography : BMC (Biomed Chromatogr) Vol. 13 Issue 1 Pg. 57-60 (Feb 1999) ISSN: 0269-3879 [Print] England
PMID10191945 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Neuraminic Acids
  • N-glycolylneuraminic acid
  • N-Acetylneuraminic Acid
Topics
  • Animals
  • Carcinoma 256, Walker (blood, metabolism)
  • Chromatography, High Pressure Liquid (methods)
  • N-Acetylneuraminic Acid (blood, metabolism)
  • Neoplasm Transplantation
  • Neuraminic Acids (blood, metabolism)
  • Rats
  • Rats, Wistar

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