Altered levels of primary antioxidant enzymes in progeria skin fibroblasts.

Abstract

Free radicals are involved in the aging process. In this study, the profile of primary antioxidant enzymes that scavenge reactive oxygen species (ROS) was examined for the first time in human skin fibroblasts from progeria, a premature aging disease. Altered levels of antioxidant enzymes were found in progeria cells. Basal levels of MnSOD were decreased in progeria cells as well as a blunted induction in response to chronic stress. This change may contribute to the accelerated aging process in progeria cells. In contrast, the levels of CuZnSOD showed no progeria-related change. Two H2O2 removing enzymes demonstrated a significant reduction in progeria cells: only 50% of normal CAT activity and 30% of normal GPX activity can be detected in progeria cells. This diminished H2O2 removing capacity in progeria cells may lead to an imbalance of intracellular ROS and therefore may play an important role in the development of progeria.

• Authors: T Yan, S Li, X Jiang, L W Oberley
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 257 Issue 1 Pg. 163-7 (Apr 02 1999) ISSN: 0006-291X [Print] United States
• PMID: 10092527 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
• Copyright: Copyright 1999 Academic Press.

Chemical References

• Antioxidants
• Reactive Oxygen Species
• Hydrogen Peroxide
• Peroxidases
• Catalase
• Glutathione Peroxidase
• Superoxide Dismutase

Topics

• Adolescent
• Aging
• Antioxidants (metabolism)
• Blotting, Western
• Catalase (metabolism)
• Cell Line
• Child
• Enzyme Induction
• Fibroblasts (enzymology, metabolism)
• Glutathione Peroxidase (metabolism)
• Humans
• Hydrogen Peroxide (metabolism)
• Male
• Matched-Pair Analysis
• Peroxidases (metabolism)
• Progeria (enzymology, metabolism)
• Reactive Oxygen Species (metabolism)
• Skin (cytology, enzymology)
• Stress, Physiological
• Superoxide Dismutase (metabolism)