Cytotoxic T-cell, delayed type hypersensitive and listeriolysin O responses in experimental bovine listeriosis.

Abstract	Five different antigens of Listeria monocytogenes NCTC 11994 were assayed for their lymphoproliferative, cytotoxic, humoral and delayed type hypersensitivity (DTH) responses in experimentally infected calves. The antigens used were culture filtrate antigen (CFA), listeriolysin O (LLO), sonicated antigen (SA), live antigen (LA) and heat killed antigen (HKA). CFA was most lymphoproliferative when assayed in vitro. In an autologous monocyte cytotoxicity assay, soluble protein as well as bacterial cells elicited cytolytic responses, however, LA and LLO primed monocytes showed a higher cytotoxic effect. The expression of cell surface markers of lymphocyte subpopulations was almost identical in experimentally infected as well as non-infected calves. SA elicited the highest humoral and DTH responses. LLO being a major virulence factor with appreciable humoral as well as cellular responses may be used as a candidate antigen to develop a reliable diagnostic immunoassay.
Authors	S B Barbuddhe, S V Malik, S Bhatnagar, L K Gupta
Journal	Veterinary microbiology (Vet Microbiol) Vol. 64 Issue 4 Pg. 333-41 (Feb 12 1999) ISSN: 0378-1135 [Print] Netherlands
PMID	10063538 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antigens, Bacterial Bacterial Toxins Heat-Shock Proteins Hemolysin Proteins hlyA protein, Listeria monocytogenes
Topics	Animals Antibody Formation (immunology) Antigens, Bacterial (blood, immunology) Bacterial Toxins Cattle Cattle Diseases (immunology, microbiology) Cytotoxicity Tests, Immunologic (veterinary) Cytotoxicity, Immunologic (immunology) Enzyme-Linked Immunosorbent Assay (veterinary) Heat-Shock Proteins (blood) Hemolysin Proteins (blood) Hypersensitivity, Delayed (immunology, veterinary) Listeria monocytogenes (immunology) Listeriosis (immunology, microbiology, veterinary) Lymphocyte Activation (immunology)

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