Transposases

Enzymes that recombine DNA segments by a process which involves the formation of a synapse between two DNA helices, the cleavage of single strands from each DNA helix and the ligation of a DNA strand from one DNA helix to the other. The resulting DNA structure is called a Holliday junction which can be resolved by DNA REPLICATION or by HOLLIDAY JUNCTION RESOLVASES.

Also Known As:

Networked: 224 relevant articles (3 outcomes, 19 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Dupuy, Adam J: 6 articles (01/2019 - 12/2008)
2.	Largaespada, David A: 6 articles (02/2015 - 08/2004)
3.	Ehrhardt, Anja: 4 articles (01/2019 - 11/2010)
4.	McIvor, R Scott: 4 articles (02/2011 - 12/2004)
5.	Hung, Chien-Fu: 3 articles (09/2021 - 01/2018)
6.	Liu, Li: 3 articles (09/2021 - 05/2006)
7.	Tseng, Ssu-Hsueh: 3 articles (09/2021 - 01/2018)
8.	Henssen, Anton G: 3 articles (10/2018 - 07/2017)
9.	Kentsis, Alex: 3 articles (10/2018 - 07/2017)
10.	Singh, Sucha: 3 articles (01/2018 - 11/2016)

Related Diseases

1.	Hypersensitivity (Allergy) 08/01/2013 - "The use of improved Sleeping Beauty transposon/transposase systems allowed us, however, to isolate DNA-PKcs-deficient iPSCs which preserved their parental genotype and hypersensitivity to ionizing radiation. " 01/01/2018 - "Background: Next-generation sequencing coupled to chromatin immunoprecipitation (ChIP-seq), DNase I hypersensitivity (DNase-seq) and the transposase-accessible chromatin assay (ATAC-seq) has generated enormous amounts of data, markedly improved our understanding of the transcriptional and epigenetic control of gene expression. " 01/01/2017 - "Chromatin immunoprecipitation, DNase I hypersensitivity and transposase-accessibility assays combined with high-throughput sequencing enable the genome-wide study of chromatin dynamics, transcription factor binding and gene regulation. " 11/01/2023 - "While initially defined by their ability to activate gene transcription in reporter assays, putative enhancer elements are now frequently defined by their unique chromatin features including DNase hypersensitivity and transposase accessibility, bidirectional enhancer RNA (eRNA) transcription, CpG hypomethylation, high H3K27ac and H3K4me1, sequence-specific transcription factor binding, and co-factor recruitment. "
2.	Inborn Genetic Diseases (Disease, Hereditary) 01/01/2015 - "Although improvements of the SB transposase systems regarding genotoxicity and efficient delivery especially for applications in large mammals are desirable, the SB transposase system remains to hold great promise for curing rare genetic disease." 01/01/2015 - "Here we will provide a state-of-the-art overview of preclinical studies for treatment of rare genetic diseases based on the SB transposase system for stable correction of the genetic defect. "
3.	Neoplasms (Cancer) 08/01/2021 - "Hepatocyte-derived liver tumors were induced in heterozygous and homozygous p53-knockout (KO) mice by hydrodynamic tail vein injection of HRAS- or Myc-containing transposon cassette plasmids, which were modified by deleting loxP sites, with a transposase-expressing plasmid. " 06/28/2017 - "Transposase-driven rearrangements in human tumors." 01/01/2016 - "We generated a rapid, cost-effective strategy to genetically engineer cancer patient T cells with TCRs using the clinical Sleeping Beauty transposon/transposase system. " 10/15/2015 - "Our results demonstrate that CRISPR/Cas9 combined with piggyBac transposase lineage labeling can produce unique models of neurodevelopmental disruption and tumors caused by somatic mutation in neural progenitors. " 10/15/2015 - "The CRISPR/Cas9 system allows for such targeted mutagenesis, and we therefore tested it in combination with a piggyBac transposase lineage labeling system to track the development of neocortical neural progenitors with targeted mutations in genes linked to neurodevelopmental disruptions and tumor formation. "
4.	Infections 01/01/2019 - "After dilution cloning, the transposon and transposase vectors were co-transfected into PK-15 cell clones with higher permissivity to PCV2 infection. " 07/01/2018 - "Molecular characterization of shrimp harbinger transposase derived 1 (HARBI1)-like and its role in white spot syndrome virus and Vibrio alginolyticus infection." 01/01/2013 - "burnetii infection, the most frequently used method is PCR and its modifications; namely, nested PCR and real time PCR which detect target sequences, such as htpAB and IS1111, chromosome genes (com1), genes specific for different types of plasmids and transposase genes. " 11/01/2006 - "pomonella larvae did not provide evidence that CpGV infection influenced the transcription level of TCp3.2 transposase." 01/01/2021 - "Using the Sleeping Beauty transposase system, we generated a robust and versatile cellular infection model that allows SARS-CoV-2 infection experiments compatible for high-throughput and live cell imaging. "
5.	Colonic Neoplasms (Colon Cancer) 01/01/2014 - "Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes." 01/01/2022 - "Methods: Here, we explored the global chromatin accessibility and transcription changes by the assay for transposase-accessible chromatin using sequencing (ATAC-seq) in combination with transcriptome sequencing of both parental and 5-FU-resistant HCT15 cells, followed by integrative analysis to better understand the regulatory network underlying 5-FU resistance in colon cancer cells. " 03/01/2021 - "The present study assessed the genetic regulatory landscape in colon cancer by performing RNA sequencing and Assay for Transposase-Accessible Chromatin sequencing, and investigated the effects of 5-FU on chromatin accessibility and gene expression. " 12/01/2013 - "OBJECTIVE-MATERIALS AND METHODS: In this particular study, it was determined the relative gene expression of Notch receptors after knockdown experiments in colon cancer stem cells (CSCs) and the gene expression changes in stemness transcription factors (Oct4, Sox2, Nanog), as well in dipeptidylpeptidase-4, CD44 antigen, Met proto-oncogene and in Metnase transposase. "

Related Drugs and Biologics

1.	Chromatin
2.	DNA (Deoxyribonucleic Acid)
3.	Deoxyribonuclease I (Nickase)
4.	Deoxyribonucleases (DNase)
5.	lysyl- arginyl- alanyl- lysyl- alanyl- lysyl- threonyl- threonyl- lysyl- lysyl- arginine (PKCS)
6.	RNA (Ribonucleic Acid)
7.	Transcription Factors (Transcription Factor)
8.	Recombinases (Recombinase)
9.	Fluorouracil (Carac)
10.	Notch Receptors

Related Therapies and Procedures

1.	Therapeutics
2.	Microbubbles
3.	Injections