Heparin Lyase (heparin lyase)

An enzyme of the isomerase class that catalyzes the eliminative cleavage of polysaccharides containing 1,4-linked D-glucuronate or L-iduronate residues and 1,4-alpha-linked 2-sulfoamino-2-deoxy-6-sulfo-D-glucose residues to give oligosaccharides with terminal 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends. (From Enzyme Nomenclature, 1992) EC 4.2.2.7.

Also Known As:

Networked: 90 relevant articles (2 outcomes, 7 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Burroughs, A K: 3 articles (02/2009 - 09/2004)
2.	Nielsen, V G: 3 articles (06/2001 - 12/2000)
3.	Cholongitas, E: 2 articles (02/2009 - 04/2007)
4.	Senzolo, M: 2 articles (02/2009 - 04/2007)
5.	Thalheimer, U: 2 articles (02/2009 - 09/2004)
6.	Jacob, Matthias: 2 articles (01/2008 - 05/2004)
7.	Welsch, Ulrich: 2 articles (01/2008 - 05/2004)
8.	Rehm, Markus: 2 articles (01/2008 - 05/2004)
9.	Conzen, Peter: 2 articles (01/2008 - 05/2004)
10.	Selinka, Hans-Christoph: 2 articles (10/2007 - 08/2002)

Related Diseases

1.	Infection 12/01/2009 - "Using an in vitro infection system, we also showed a marked reduction in HEV infection of heparinase-treated cells. " 01/01/2008 - "Pretreatment of primary hepatocytes with heparinase decreased viral binding and inhibited HBV infection completely. " 04/01/2004 - "tsutsugamushi infection decreased markedly after treating host cells with heparinase, which suggests that HSPGs play an important role in oriential infection. " 04/10/2001 - "(ii) Enzymatic removal of HFF cell surface HS with heparinase I and III reduced HHV-8 infection. " 10/01/2000 - "Removal of HS from the surface of swine kidney cells (SK6) by heparinase I treatment almost completely abolished infection of these cells with virus that was extensively passaged in swine kidney cells before it was cloned (clone C1.1.1). "
2.	Fibrosis (Cirrhosis) 05/01/2009 - "HLE was defined as a correction of r+k times on TEG of more than 50% by the addition of heparinase I. Before incision, patients with ALF showed a significantly greater HLE compared with patients with cirrhosis (r+k time: 66 min corrected to 29 vs 45 min corrected to 32 min, P=0.001). " 02/01/2009 - "The role of endogenous heparinoids (glycosaminoglycans) in the coagulopathy of patients who have cirrhosis has been demonstrated by thromboelastography with the addition of heparinase I in patients who have recent variceal bleeding and infection. "
3.	Hemorrhage 10/01/2002 - "This study suggests that timing and the use of heparinase influence the predictive ability of thromboelastography, but its usefulness as a sole predictor of post-CABG surgery bleeding is limited." 06/01/2001 - "However, blood samples exposed to heparinase after hemorrhage demonstrated enhanced hemostatic function with thrombelastographic values (R = 13.4 +/- 1.5, alpha 56.0 +/- 3.4, G = 7012 +/- 565) significantly different (P <.05) from samples not exposed to heparinase. " 06/01/2001 - "Thrombelastographic variable values in samples without platelet inhibition or heparinase exposure did not significantly change after hemorrhage (before hemorrhage: R = 22.01 +/- 0.7 min, alpha 43.6 +/- 1.3 degrees, G 7,089 +/- 379 dyne/cm(2); after hemorrhage: R 22.1 +/- 2.4, alpha 41.6 +/- 3.9, G = 5,662 +/- 564; mean +/- SEM). " 07/01/1995 - "Treatment of excessive bleeding after cardiopulmonary bypass was based on an algorithm using point-of-care testing with whole blood prothrombin time, activated partial thromboplastin time, heparinase activated clotting time, and platelet count. " 01/01/2001 - "In the study group, celite activated heparinase pretreated blood samples, 30 minutes after protamine administration were subjected to thromboelastographic analysis and blood and blood component therapy was administered based on thromboelastograph values, if they had significant bleeding. "
4.	Hemolysis 06/05/1999 - "In a previous study, we showed that the fluidization of agarose immobilized heparinase within Taylor vortices in whole blood can lead to extensive blood damage in the form of cell depletion and hemolysis. " 05/01/2006 - "Results from degradation studies indicated that the crosslinker was sensitive to collagenase type I but not to thrombin or heparinase I, and a hemolysis assay suggested that the 2:1 gels and 3:1 materials might be biocompatible. "
5.	Neoplasms (Cancer) 08/01/2003 - "By contrast, the tumors treated with heparinase or grown in the NDST-2 knockout mice were significantly larger than the tumors from control (untreated) mice. " 02/01/2006 - "Overexpression of heparinase, when coupled with loss of CD138 expression, may take part in tumor metastasis of HCC." 11/01/2013 - "The presence of a glycocalyx-like layer was verified around tumor cells, and the degradation of this layer by hyaluronidase and heparinase blocked the flow-regulated invasion. " 04/01/2011 - "A thromboelastogram performed with heparinase I digestion demonstrated the action of endogenous heparin-like substances whose presence was subsequently confirmed at the histological examination of the tumor. " 09/20/2003 - "Accelerated growth and blood clotting were also observed in tumor-bearing mice treated with heparinase I enzyme to destroy endogenous mast cell heparin and in NDST-2 knockout mice in which there is a targeted disruption in the gene coding for mast cell heparin synthesis. "

Related Drugs and Biologics

1.	Heparin (Liquaemin)
2.	Glycosaminoglycans
3.	heparanase
4.	Thrombin
5.	Sepharose (Agarose)
6.	Collagenases (Collagenase)
7.	HLA-A2 Antigen (HLA A2 Antigen)
8.	Diatomaceous Earth (Kieselguhr)
9.	Interleukin-2 (IL2)
10.	Gels

Related Therapies and Procedures

1.	Cardiopulmonary Bypass (Heart-Lung Bypass)
2.	Transplants (Transplant)
3.	Liver Transplantation
4.	Coronary Artery Bypass (Coronary Artery Bypass Surgery)
5.	Aftercare (After-Treatment)