Endopeptidase K (Proteinase K)

An enzyme that catalyzes the hydrolysis of keratin, and of other proteins with subtilisin-like specificity. It hydrolyses peptide amides. Endopeptidase K is from the mold Tritirachium album Limber. (Enzyme Nomenclature, 1992) EC 3.4.21.64.

Also Known As:

Networked: 374 relevant articles (7 outcomes, 25 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Gambetti, Pierluigi: 9 articles (01/2018 - 10/2003)
2.	Shi, Qi: 6 articles (09/2021 - 07/2008)
3.	Zou, Wen-Quan: 6 articles (04/2021 - 10/2003)
4.	Cali, Ignazio: 6 articles (01/2021 - 11/2006)
5.	Dong, Xiao-Ping: 6 articles (01/2017 - 08/2005)
6.	Kretzschmar, Hans A: 6 articles (11/2007 - 03/2002)
7.	Andréoletti, Olivier: 5 articles (06/2021 - 03/2008)
8.	Ironside, James W: 5 articles (01/2020 - 03/2008)
9.	Han, Jun: 5 articles (01/2017 - 08/2005)
10.	Zhang, Bao-Yun: 5 articles (01/2017 - 08/2005)

Related Diseases

1.	Scrapie 10/06/2016 - "Furthermore scrapie samples from goats in the current study demonstrated less long term resistance to proteinase K than ovine or caprine BSE control samples. " 01/15/2007 - "We have developed a CDI (conformation-dependent immunoassay) utilizing time-resolved fluorescence to study the conformers of disease-associated PrP in natural cases of sheep scrapie, without using PK (proteinase K) treatment to discriminate between PrPC and PrPSc. " 06/01/1999 - "In this study, the long-term proteinase K resistance, the molecular mass, and the localization of PrP(Sc) deposits derived from conventional and transgenic mice inoculated with 11 different BSE and scrapie strains or isolates were examined. " 11/29/2016 - "Remarkably, even though fibrils in the inoculum lack the entire C-terminal domain of PrP, brains of clinically sick mice accumulate longer proteinase K-resistant (PrPres) fragments of ∼17-32 kDa, similar to those observed in classical scrapie strains. " 12/06/2013 - "Infrared microspectroscopy revealed that PMCA of native hamster 263K scrapie seeds in hamster PrP(C) substrate caused conformational alterations in progeny seeds that were accompanied by an altered resistance to Proteinase K, higher sedimentation velocities in gradient ultracentrifugations, and a longer incubation time in animal bioassays. "
2.	Prion Diseases (Transmissible Spongiform Encephalopathies) 01/01/2017 - "Susceptibility to sporadic prion disease correlates well with the propensity of PrP to misfold to cytotoxic, proteinase K resistant oligomeric conformations at low pH. In this study, mutagenesis at the hydrophobic core of the mouse PrP has been shown to stabilize a monomeric unfolding intermediate (I), which is populated significantly at equilibrium at low pH. Importantly, the rate of formation of β-structured oligomers at low pH is found to correlate well with the extent to which this intermediate is populated. " 09/01/2013 - "The purpose of this study was to investigate three things: the possible mechanisms of neuron cell damage, the conformation of anti-protease K (PK) PrP(Sc), and the role of oxidative stress in the progression of prion diseases. " 09/01/2019 - "Neuropathological aspects of prion disease were studied by histological analysis and proteinase K digestion. " 08/01/2014 - "Prion diseases, which can manifest by transmissible, sporadic or genetic etiologies, share several common features, such as a fatal neurodegenerative outcome and the aberrant accumulation of proteinase K (PK)-resistant PrP forms in the CNS. " 11/01/2013 - "A number of disease-associated PrP forms characterized by abnormally short proteinase K-resistant fragments (atypical PrPres) were recently described in prion diseases. "
3.	Acne Vulgaris 01/01/2023 - "Proteinase K/Retinoic Acid-Loaded Cationic Liposomes as Multifunctional Anti-Acne Therapy to Disorganize Biofilm and Regulate Keratinocyte Proliferation." 01/01/2023 - "In this study, we present novel anti-acne nanovesicles based on liposomes loaded with proteinase K (PK), retinoic acid (RA), and soyaethyl morpholinium ethosulfate (SME) to achieve an effective and safe treatment. "
4.	Cysts 02/21/2012 - "A significant reduction in attachment was observed following both cyst treatments with proteinase K and performing experiments at extremes of pH (2 and 12). " 04/01/1991 - "With the most efficient procedure, disrupting Giardia cysts with glass beads in the presence of proteinase K, as few as 1 to 5 cysts per ml can be detected in water sample concentrates with dot blot hybridization assays." 02/21/2012 - "It is suggested that proteinase K removes the proteins needed for specific surface interactions, whereas extremes of pH influence either protonation of the polymer or the surface charge of the cysts. " 06/03/2022 - "DNA extraction was performed in a volume of 20 μl containing 3 μl 10× PCR buffer, 3 μl Proteinase K (600 μg μl-1), 14 μl distilled water and a single cyst was added and ground in an ice bath as described by Ou et al. (2008). " 06/01/2013 - "DNA was extracted by putting a single cracked cyst collected from the tobacco root to a 0.2-ml Eppendorf tube containing 10 μl double distilled water, 8 μl 10 × PCR Buffer (Mg-), and 2 μl of proteinase K (600 μg/ml) and frozen at -70°C for 30 min, then incubated at 65°C for 1 h and at 95°C for 10 min. After centrifugation at 12,000 rpm for 2 min, the DNA suspension was used for PCR amplification. "
5.	Metrorrhagia (Spotting) 01/01/1992 - "The test proved valid for detection of BECV in the supernatant of infected HRT-18 cells: genomic RNA could be detected after direct spotting of samples, but prior nucleic acid extraction after proteinase K treatment improved virus detection. " 12/01/1989 - "A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. "

Related Drugs and Biologics

1.	Prion Proteins
2.	RNA (Ribonucleic Acid)
3.	Liposomes (Liposome)
4.	Tretinoin (Retinoic Acid)
5.	soyaethyl morpholinium
6.	Nucleic Acids
7.	Sodium Dodecyl Sulfate (Sodium Lauryl Sulfate)
8.	Subtilisins (Subtilopeptidase A)
9.	sodium percarbonate
10.	Proteins (Proteins, Gene)

Related Therapies and Procedures

1.	Oral Administration
2.	Therapeutics
3.	Prostheses and Implants (Prosthesis)
4.	Catheters
5.	Photochemotherapy (Photodynamic Therapy)