Endopeptidase K (Proteinase K)

An enzyme that catalyzes the hydrolysis of keratin, and of other proteins with subtilisin-like specificity. It hydrolyses peptide amides. Endopeptidase K is from the mold Tritirachium album Limber. (Enzyme Nomenclature, 1992) EC 3.4.21.64.

Also Known As:

Networked: 281 relevant articles (4 outcomes, 18 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Gambetti, Pierluigi: 6 articles (03/2013 - 10/2003)
2.	Zou, Wen-Quan: 5 articles (03/2013 - 10/2003)
3.	Beekes, Michael: 4 articles (12/2013 - 08/2004)
4.	Groschup, Martin H: 4 articles (10/2011 - 08/2003)
5.	Kretzschmar, Hans A: 4 articles (08/2007 - 03/2002)
6.	Sy, Man-Sun: 4 articles (05/2006 - 04/2003)
7.	Li, Ruliang: 4 articles (01/2005 - 01/2002)
8.	Kovacs, Gabor G: 3 articles (11/2013 - 03/2009)
9.	Kong, Qingzhong: 3 articles (03/2013 - 11/2006)
10.	Yuan, Jue: 3 articles (03/2013 - 11/2006)

Related Diseases

1.	Cysts 02/21/2012 - "A significant reduction in attachment was observed following both cyst treatments with proteinase K and performing experiments at extremes of pH (2 and 12). " 04/01/1991 - "With the most efficient procedure, disrupting Giardia cysts with glass beads in the presence of proteinase K, as few as 1 to 5 cysts per ml can be detected in water sample concentrates with dot blot hybridization assays." 02/21/2012 - "It is suggested that proteinase K removes the proteins needed for specific surface interactions, whereas extremes of pH influence either protonation of the polymer or the surface charge of the cysts. " 01/01/2008 - "Acanthamoeba cysts mixed with a tag virus were processed according to different DNA preparation procedures: heat, Proteinase K (ProtK), alkali lysis, QIAmp kit, MagNA Pure (DNA Mini kit, MagNA Pure Nucleic Acid isolation kit), ProtK+QIAmp and ProtK+MagNA Pure. " 08/01/2009 - "Cyst walls were prepared by removing intracellular material from cysts by pre-treating them with sodium dodecyl sulphate (SDS) containing dithiothreitol, and then subjecting these to a series of sequential enzymatic digestions using amyloglucosidase, papain, DNase, RNase and proteinase K. "
2.	Body Weight (Weight, Body) 11/01/2013 - "Oral administration (100mg/kg body weight) of the RPHs and RPI to spontaneously hypertensive rats (SHR) showed the Alcalase RPH to be the most effective in blood pressure (BP) reduction (∼24 mm Hg) while Proteinase K RPH was the least effective (∼5 mm Hg) after 8h. " 12/01/1997 - "Rats treated with daily injection of DL-isoproterenol for 10 consecutive days (25 mg kg(-1) body weight) showed marked induction of a proline-rich glycoprotein (GPRP) of 220 kDa. Proteinase K digestion of GPRP produced a homogeneous glycopeptide with an average chemical composition as follows (residues per mol): Pro4, Glx3, Asx2, Gly1, His1, Thr1, Arg1, GlcNAc5, GalNac1, Man3, Gal2-3, and Fuc1. "
3.	Pleuropneumonia 03/01/1991 - "Improved protection of swine from pleuropneumonia by vaccination with proteinase K-treated outer membrane of Actinobacillus (Haemophilus) pleuropneumoniae."
4.	Metrorrhagia (Spotting) 01/01/1992 - "The test proved valid for detection of BECV in the supernatant of infected HRT-18 cells: genomic RNA could be detected after direct spotting of samples, but prior nucleic acid extraction after proteinase K treatment improved virus detection. "
5.	Scrapie 10/01/2008 - "In this study, we subjected prion-infected brain homogenates derived from three different hamster scrapie strains to either (i) proteinase K digestion or (ii) sonication, and inoculated the modified samples into normal hamsters. " 01/15/2007 - "We have developed a CDI (conformation-dependent immunoassay) utilizing time-resolved fluorescence to study the conformers of disease-associated PrP in natural cases of sheep scrapie, without using PK (proteinase K) treatment to discriminate between PrPC and PrPSc. " 06/01/1999 - "In this study, the long-term proteinase K resistance, the molecular mass, and the localization of PrP(Sc) deposits derived from conventional and transgenic mice inoculated with 11 different BSE and scrapie strains or isolates were examined. " 01/01/2014 - "Here we show that, by applying pressures at kbar range, the "proteinase K-resistant" fibrils (rHaPrP(res)) prepared from hamster prion protein (rHaPrP [23-231]) by seeding with brain homogenate of scrapie-infected hamster, becomes easily digestible. " 01/01/2014 - "Pressure-assisted dissociation and degradation of "proteinase K-resistant" fibrils prepared by seeding with scrapie-infected hamster prion protein."

Related Drugs and Biologics

1.	RNA (Ribonucleic Acid)
2.	Subtilisins (Subtilopeptidase A)
3.	DNA (Deoxyribonucleic Acid)
4.	Proteins (Proteins, Gene)
5.	Trypsin
6.	Prions
7.	Ubiquitin
8.	sarkosyl
9.	Pancreatin
10.	Mannose (D-Mannose)

Related Therapies and Procedures

1.	Oral Administration
2.	Prostheses and Implants (Prosthesis)