DNA Primers

Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

Also Known As:

Primer, DNA; Primer, Oligodeoxyribonucleotide; Primer, Oligonucleotide; Primers, DNA; Primers, Oligodeoxyribonucleotide; Primers, Oligonucleotide; DNA Primer; Oligodeoxyribonucleotide Primer; Oligodeoxyribonucleotide Primers; Oligonucleotide Primer; Oligonucleotide Primers

Networked: 240 relevant articles (1 outcomes, 13 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Zhang, H: 2 articles (02/2021 - 12/2001)
2.	Pang, Yaoshan: 2 articles (01/2008 - 07/2002)
3.	Xie, Zhixun: 2 articles (01/2008 - 07/2002)
4.	Baudis, Michael: 2 articles (05/2006 - 07/2005)
5.	Hamoudi, Rifat A: 2 articles (05/2006 - 07/2005)
6.	Mao, Xin: 2 articles (05/2006 - 07/2005)
7.	Abliz, Paride: 2 articles (01/2004 - 06/2003)
8.	Fukushima, Kazutaka: 2 articles (01/2004 - 06/2003)
9.	Nishimura, Kazuko: 2 articles (01/2004 - 06/2003)
10.	Takizawa, Kayoko: 2 articles (01/2004 - 06/2003)

Related Diseases

1.	Infections 04/30/1999 - "cruzi infection, 17 gave positive results when genomic DNA primers were used. " 03/01/2023 - "pusillus infection was diagnosed using an in-house panfungal PCR/sequencing assay based on dual priming oligonucleotide primers. " 01/01/2020 - "Comprehensive surveillance, including virus isolation, real-time reverse transcription-polymerase chain reaction (RT-PCR), and consequential degenerate hybrid oligonucleotide primer (CODEHOP) methods, were arranged for specimens (rectal or throat swabs), which were obtained from all contacts, newly admitted cases, and suspected cases during the outbreak since June 2. Ten cases were identified with echovirus 11 infection in this outbreak. " 03/01/2005 - "Several oligonucleotide primer pairs, based on the KTSV genomic sequence, were used to efficiently detect the virus in plants, thereby removing a major constraint to reliable screening of kalanchoë propagating stock and breeding lines for KTSV infection. " 10/01/2004 - "The PCR results obtained from PCV2 specific oligonucleotide primers confirmed a PCV2 infection. "
2.	Neoplasms (Cancer) 01/01/2018 - "Students were first introduced to the bioinformatics tools to identify appropriate DNA primers to amplify specific pharmacogenes from the laboratory cancer cell lines. " 05/01/2015 - "Quantitative polymerase chain reaction analysis with allele-specific oligonucleotide primers for individual IgH VDJ regions to evaluate tumor burden in myeloma patients." 04/15/2000 - "Nested PCR with patient IgH allele-specific oligonucleotide primers can detect 1 tumor cell in 10(4) to 10(6) normal cells. " 02/15/1997 - "Differential gene expression of candidate informative markers between cancer and benign was screened by the polymerase chain reaction with gene-specific oligonucleotide primers. " 01/01/1997 - "Using specific oligonucleotide primers for the nm23-H1 microsatellite repetitive sequence, gene instability was followed by polymerase chain reaction/loss of heterozygosity assay on 54 tumor specimens and the corresponding normal tissue samples. "
3.	Malaria 12/01/1996 - "Therefore, a cross-sectional study was conducted in the districts of Kabarole and Bundibugyo in western Uganda using material from three villages with different epidemiologic situations regarding malaria and DNA primers for a PCR that had shown satisfactory sensitivity and specificity in previous trials. " 04/01/1993 - "Oligonucleotide primer pairs specific to each species were designed for specific amplification by the Polymerase Chain Reaction (PCR), to detect each malaria species. " 03/01/2003 - "Peptide sequences were obtained from purified RhopH2 proteins and used to generate oligonucleotide primers and search malaria sequence databases. " 12/15/1994 - "Degenerate oligonucleotide primers were designed for conserved regions of cytochrome P450 proteins of the CYP4 family and were used to amplify cDNA or genomic DNA from different strains of the New World malaria vector, Anopheles albimanus (Weidemann). "
4.	Ovarian Neoplasms (Ovarian Cancer) 01/31/2011 - "In this study, we performed cDNA subtraction following polymerase chain reaction (PCR) using degenerate oligonucleotide primers to identify specifically overexpressed PTKs in ovarian cancer. " 05/01/1996 - "To begin to assess the role of PTPs in ovarian cancer cell growth regulation, PTP partial cDNAs were cloned from the OVCA 433 ovarian cancer cell line using RT/PCR and degenerate oligonucleotide primers for the PTP consensus domain. " 05/01/1996 - "An oligonucleotide primer specific for a conserved amino acid region of the Cys2/His2 zinc finger proteins was used in conjunction with mRNA differential display to amplify related mRNAs from a human ovarian cancer cell line. "
5.	Breast Neoplasms (Breast Cancer) 07/15/2005 - "To address this issue, we performed allelotyping analysis of formalin-fixed, paraffin-embedded samples from 21 Chinese patients with breast cancer using 59 fluorescently tagged oligonucleotide primers amplifying microsatellite loci. " 04/01/1993 - "RNA from breast tumors and normal breast tissue was reverse transcribed and then amplified using oligonucleotide primers for human aromatase or KGF. " 05/15/1991 - "The GnRH gene is expressed in these breast tumor lines, as determined by S1 nuclease protection assay, oligonucleotide primer extension studies, and polymerase chain reaction amplification of complementary DNA using oligonucleotides. "

Related Drugs and Biologics

1.	Complementary DNA (cDNA)
2.	DNA (Deoxyribonucleic Acid)
3.	Messenger RNA (mRNA)
4.	RNA-Directed DNA Polymerase (Reverse Transcriptase)
5.	RNA (Ribonucleic Acid)
6.	Enzymes
7.	Transforming Growth Factor beta1 (TGF beta 1)
8.	Transforming Growth Factor beta (TGF-beta)
9.	Transforming Growth Factor alpha (TGF-alpha)
10.	Transforming Growth Factors (Transforming Growth Factor)

Related Therapies and Procedures

1.	Therapeutics
2.	Surgical Portacaval Shunt (Portacaval Anastomosis)
3.	Ligation
4.	Lasers (Laser)