DNA Primers

Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

Also Known As:

Networked: 197 relevant articles (1 outcomes, 8 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Xie, Zhixun: 2 articles (01/2008 - 07/2002)
2.	Pang, Yaoshan: 2 articles (01/2008 - 07/2002)
3.	Baudis, Michael: 2 articles (05/2006 - 07/2005)
4.	Mao, Xin: 2 articles (05/2006 - 07/2005)
5.	Hamoudi, Rifat A: 2 articles (05/2006 - 07/2005)
6.	Abliz, Paride: 2 articles (01/2004 - 06/2003)
7.	Nishimura, Kazuko: 2 articles (01/2004 - 06/2003)
8.	Takizawa, Kayoko: 2 articles (01/2004 - 06/2003)
9.	Fukushima, Kazutaka: 2 articles (01/2004 - 06/2003)
10.	Iida, Masato: 1 article (05/2015)

Related Diseases

1.	Infection 04/30/1999 - "cruzi infection, 17 gave positive results when genomic DNA primers were used. " 10/01/2004 - "The PCR results obtained from PCV2 specific oligonucleotide primers confirmed a PCV2 infection. " 08/01/2001 - "Infection is detected by a single multiplex PCR reaction containing two pairs of oligonucleotide primers whereby each primer is specific for each parasite species. " 02/01/2000 - "Early brown rot infections in sweet cherry fruit are detected by monilinia-specific DNA primers." 11/01/1995 - "The prevalence of genotype distribution of HCV infection in Taiwan was investigated by typing with type-specific DNA primers in HCV core region. "
2.	Neoplasms (Cancer) 05/01/2015 - "Quantitative polymerase chain reaction analysis with allele-specific oligonucleotide primers for individual IgH VDJ regions to evaluate tumor burden in myeloma patients." 01/01/2004 - "HPV was detected in 95.5% of invasive cervical cancers, in 91.6% of high grade lesions, in 66.7% of low grade lesions, and in 23.1% of normal smears, by PCR with at least one set of oligonucleotide primers. " 04/15/2000 - "Nested PCR with patient IgH allele-specific oligonucleotide primers can detect 1 tumor cell in 10(4) to 10(6) normal cells. " 02/15/1997 - "Differential gene expression of candidate informative markers between cancer and benign was screened by the polymerase chain reaction with gene-specific oligonucleotide primers. " 01/01/1997 - "Using specific oligonucleotide primers for the nm23-H1 microsatellite repetitive sequence, gene instability was followed by polymerase chain reaction/loss of heterozygosity assay on 54 tumor specimens and the corresponding normal tissue samples. "
3.	Malaria 12/01/1996 - "Therefore, a cross-sectional study was conducted in the districts of Kabarole and Bundibugyo in western Uganda using material from three villages with different epidemiologic situations regarding malaria and DNA primers for a PCR that had shown satisfactory sensitivity and specificity in previous trials. " 03/01/2003 - "Peptide sequences were obtained from purified RhopH2 proteins and used to generate oligonucleotide primers and search malaria sequence databases. " 12/15/1994 - "Degenerate oligonucleotide primers were designed for conserved regions of cytochrome P450 proteins of the CYP4 family and were used to amplify cDNA or genomic DNA from different strains of the New World malaria vector, Anopheles albimanus (Weidemann). "
4.	Ovarian Neoplasms (Ovarian Cancer) 01/01/2011 - "In this study, we performed cDNA subtraction following polymerase chain reaction (PCR) using degenerate oligonucleotide primers to identify specifically overexpressed PTKs in ovarian cancer. " 05/01/1996 - "To begin to assess the role of PTPs in ovarian cancer cell growth regulation, PTP partial cDNAs were cloned from the OVCA 433 ovarian cancer cell line using RT/PCR and degenerate oligonucleotide primers for the PTP consensus domain. "
5.	Glucose Intolerance 01/01/1990 - "The sequences of the gene and oligonucleotide primers will facilitate studies of genetic variation in the hINSR gene and thereby increase our understanding of the role of this gene in the development of insulin-resistant states and glucose intolerance."

Related Drugs and Biologics

1.	Complementary DNA (cDNA)
2.	Messenger RNA (mRNA)
3.	RNA-Directed DNA Polymerase (Reverse Transcriptase)
4.	DNA (Deoxyribonucleic Acid)
5.	RNA (Ribonucleic Acid)
6.	Enzymes
7.	Transforming Growth Factor beta1 (TGF beta 1)
8.	Transforming Growth Factor beta (TGF-beta)
9.	Transforming Growth Factor alpha (TGF-alpha)
10.	Transforming Growth Factors (Transforming Growth Factor)

Related Therapies and Procedures

1.	Heterologous Transplantation (Xenotransplantation)
2.	Surgical Portacaval Shunt (Portacaval Anastomosis)
3.	Ligation