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Serum-Free Culture Media

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CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.

Also Known As:

Culture Media, Serum-Free; Low-Serum Media; Culture Media, Serum Free; Low Serum Media; Media, Low-Serum; Media, Protein-Free; Media, Serum-Free; Media, Serum-Free Culture; Protein Free Media; Serum Free Media; Protein-Free Media; Serum-Free Media

Networked: 193 relevant articles (2 outcomes, 11 trials/studies)

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Bio-Agent Context: Research Results

Experts

1.	Zheng, Ai: 2 articles (09/2014 - 01/2011)
2.	Lloyd, Ricardo V: 2 articles (09/2013 - 03/2013)
3.	Hardin, Heather: 2 articles (09/2013 - 03/2013)
4.	Montemayor-Garcia, Celina: 2 articles (09/2013 - 03/2013)
5.	Chen, Qi: 2 articles (11/2007 - 04/2007)
6.	Jeong, Hye-Cheol: 1 article (01/2016)
7.	Kim, Gwangil: 1 article (01/2016)
8.	Lee, Mi-Jung: 1 article (01/2016)
9.	Lee, Ji-Hyun: 1 article (01/2016)
10.	Jeong, Ju-Yeon: 1 article (01/2016)

Related Diseases

1.	Neoplasms (Cancer) 04/15/2013 - "GFP-bright cells had the ability to form more tumor spheres in serum-free media and were able to generate both GFP-bright and GFP-dim (lower Notch activity) cell populations. " 01/01/2013 - "In this chapter we wish to broadly outline the process of establishing, characterizing, and managing novel cancer cell lines in defined serum-free media, and discuss the limitations and potential opportunities that may arise from these model systems." 01/01/2011 - "Human cervical cancer cell lines (HeLa cells) were incubated with serum-free media for 24 hours. " 12/01/2010 - "Tumor stem cell enrichment from primary tumor derived cell spheres has been demonstrated in specific, serum-free media. " 05/01/2008 - "IGF-IR overexpression provided the primary tumor cells with a survival advantage in serum-free media and seemed to induce ligand-independent activation of the IGF-IR because RM11A cells cultured in the presence of Dox were largely nonresponsive to exogenous IGFs. "
2.	Melanoma (Melanoma, Malignant) 07/01/2015 - "Most of the melanoma lines, including WM35, FEMX and A375, can grow as a spheroid culture in serum-free media with supplements. " 05/01/2015 - "Melanoma cells form monolayers in serum-containing media, however, in serum-free media they form anchorage-independent spheroids. " 01/01/1992 - "A nonrecombinant human melanoma cell line and recombinant chinese hamster ovary (CHO) cells were used as examples for long-term in vitro cultivation in protein-free media. " 01/15/1986 - "The growth properties of a human melanoma cell line (MeWo) and of a variant (MeWo-LC1) endowed with higher metastatic potential in nude mice were compared using hormonally-defined serum-free media. " 06/01/1982 - "[Establishment and characterization of human melanoma cells continuously grown in serum-free media]."
3.	Carcinoma (Carcinomatosis) 03/01/2012 - "Establishment of human ovarian serous carcinomas cell lines in serum free media." 11/01/1995 - "The KB oral carcinoma cell line was studied in serum-free media. " 07/01/1990 - "Improved serum-free media were developed for the anchorage-dependent growth of A549 human lung carcinoma and B16 mouse melanoma cell lines in vitro. " 07/01/1993 - "Human fibroblast, bladder carcinoma, and breast carcinoma cells in commercial serum-free media or balanced salt solutions rapidly lose K+ and gain Na+. This rapid K+ loss is caused by one or more impurities in phenol red. " 06/01/1991 - "Primary lung carcinomas were established from explant outgrowths and maintained in serum supplemented or serum free media on plastic or basement membrane associated protein coated dishes in order to obtain cells for karyotypic analysis (Miura et al., 1990). "
4.	Neuroblastoma 02/01/2014 - "Newly-derived neuroblastoma cell lines propagated in serum-free media recapitulate the genotype and phenotype of primary neuroblastoma tumours." 01/01/2006 - "Neuroblastoma cell lines (SK-N-AS, SK-N-DZ, and SK-N-FI) were grown in serum-free media. " 01/01/2001 - "Esterase inhibition was determined in SH-SY5Y human neuroblastoma cells grown in serum-free media and exposed to 10(-11) to 10(-7) M concentrations of organophosphorus (OP) compounds for 28 days. " 02/01/1996 - "1. We report that NG108-15 (neuroblastoma x glioma) cells differentiated in defined serum-free media are capable of exhibiting stable automaticity (the spontaneous occurrence of regenerative action potentials) following exposure to extracellular perfusates containing NH4Cl. " 12/24/1993 - "The electrophysiological properties of NG108-15 neuroblastoma x glioma hybrids were compared after culture in serum-containing medium (SCM) versus serum-free media (SFM) containing N2 or B27 supplements. "
5.	Glioma (Gliomas) 05/05/2012 - "This study describes a detailed process for obtaining brain glioma stem cells from freshly dissected human brain glioma samples using an immunomagnetic bead technique combined with serum-free media pressure screening. " 08/01/1994 - "In C6 rat glioma cells, the addition of E2 to serum free media enhances live cell number by 40% at 24 h and 75% at 96 h when compared to serum free media conditions. " 10/30/1990 - "The isolation of a metalloproteinase secreted by a rat glioma cell line (BT5C) in serum-free media is described. " 05/05/2012 - "Results showed that a small number of CD133 positive tumor cells isolated from brain glioma samples survived as a cell suspension in serum-free media and proliferated. " 02/01/1996 - "1. We report that NG108-15 (neuroblastoma x glioma) cells differentiated in defined serum-free media are capable of exhibiting stable automaticity (the spontaneous occurrence of regenerative action potentials) following exposure to extracellular perfusates containing NH4Cl. "

Related Drugs and Biologics

1.	Indicators and Reagents (Reagents)
2.	Insulin (Novolin)
3.	DNA (Deoxyribonucleic Acid)
4.	Collagenases (Collagenase)
5.	Sepharose (Agarose)
6.	Phosphatidylinositol 3-Kinase (1 Phosphatidylinositol 3 Kinase)
7.	Insulin Receptor Substrate Proteins
8.	Cytokines
9.	Insulin Receptor
10.	Polytetrafluoroethylene (Teflon)

Related Therapies and Procedures

1.	Microspheres (Microsphere)
2.	Heterologous Transplantation (Xenotransplantation)
3.	Solid-State Lasers
4.	Transplants (Transplant)
5.	Immunotherapy