Fluorescein-5-isothiocyanate (FITC)

Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Also Known As:

FITC; Fluorescein 5 isothiocyanate; 5-Isothiocyanatofluorescein; Fluorescein (5 or 6)-Isothiocyanate; Fluorescein-5-isothiocyanate Hydrochloride; 5 Isothiocyanatofluorescein; Fluorescein 5 isothiocyanate Hydrochloride; Hydrochloride, Fluorescein-5-isothiocyanate; Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-5-isothiocyanato-

Networked: 1239 relevant articles (24 outcomes, 112 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Imai, Yasuyuki: 14 articles (05/2020 - 01/2007)
2.	Kurohane, Kohta: 14 articles (05/2020 - 01/2007)
3.	Low, Philip S: 7 articles (01/2019 - 01/2006)
4.	Hong, Min: 5 articles (02/2019 - 06/2016)
5.	Wang, Xiaoyu: 5 articles (02/2019 - 08/2015)
6.	Moore, Bethany B: 5 articles (01/2018 - 04/2004)
7.	Baker, James R: 5 articles (04/2011 - 01/2005)
8.	Liu, Shuang: 4 articles (11/2021 - 08/2012)
9.	Bielke, L R: 4 articles (09/2021 - 06/2015)
10.	Matsuoka, Takeshi: 4 articles (05/2020 - 01/2015)

Related Diseases

1.	Neoplasms (Cancer) 01/01/2016 - "We demonstrate that both switch formats can be readily optimized to redirect CAR-T cells (specific for the corresponding FITC or PNE) to Her2-expressing tumor cells, and afford dose-titratable activation of CAR-T cells ex vivo and complete clearance of the tumor in rodent xenograft models. " 01/01/2020 - "Our results demonstrated that Aln-FITC might have the potential for minimally invasive and real-time in vivo bone pH sensing in preclinical studies of bone healing, metabolism, and cancer mechanisms." 08/01/2017 - "Moreover, proapoptotic and antiproliferative studies showed that Fe3O4@SiO2(FITC)-BTN/QUR/DOX nanoparticles can promote apoptosis, inhibit tumor cell proliferation, and enhance the chemotherapeutic effects of DOX against multidrug resistance. " 01/01/2015 - "Fluorescence microscopic studies indicated that the FITC-labeled K4R2-Nal2-S1 preferentially binds cancer cells and causes apoptotic cell death. " 04/01/2010 - "In vivo imaging study showed that FITC-labeled Ec-LDP-Hr was targeted and accumulated in the tumors. "
2.	Inflammation (Inflammations) 01/01/2016 - "Synthetic BK or mBK labeled with FITC at the N-terminus was employed for discriminating between inflamed and normal cells; this method was found to be effective for detection of inflammation in live cells. " 01/01/2015 - "The present study evaluated different methods to induce gut inflammation in poultry based on FITC-d leakage. " 01/01/2017 - "Here, we describe in detail how inflammation can be induced in the skin with tape stripping or FITC painting and how the skin DC network can be monitored using immunofluorescence microscopy and flow cytometry." 06/01/2015 - "All inflammation treatments significantly increased serum FITC-d levels at both doses. " 06/01/2015 - "Dose titration of FITC-D for optimal measurement of enteric inflammation in broiler chicks."
3.	Ischemia 09/01/1989 - "After determination of baseline data, the muscle was made ischemic for 2 hr by clamping its vascular pedicle, and subsequently reperfused for 2 hr. Ischemia-reperfusion produced a marked increase in clearance of FITC-Dx 150. " 07/01/1995 - "In saline-treated animals, ischemia-reperfusion increased the clearance of FITC-Dx 150 from 1.8 +/- 0.3 to 9.7 +/- 1.0 microliters/15 min/g by the end of the reperfusion period. " 07/01/1995 - "After determination of baseline data, the muscle was made ischemic for 4 hr by clamping the vascular pedicle and subsequently reperfused for 2 hr. In control animals not subjected to ischemia, clearance of FITC-Dx 150 remained constant throughout the experimental 7-hr period. " 05/01/1987 - "Clearance of FITC-Dx 150 increased from a control value (mean +/- SE) of 8.3 +/- 2.7 to 29.9 +/- 8.1 microliters/min/g after reperfusion following a 30-min period of ischemia, and from a control value of 36.2 +/- 13.6 to 274 +/- 94.5 after 2 hr of ischemia. " 02/01/1997 - "Laser confocal microscope demonstrated that the FITC quantum on blood vessels after reperfusion was more than 47% compared to that of pure ischemia. "
4.	Thrombosis (Thrombus) 12/01/2009 - "We first synthesized FITC-KGDS-Palm compound, and then prepared thrombus-targeted microbubbles using "ultrasound & high speed shearing method". " 11/15/2020 - "The results of FITC labeling illustrated that hAvHA bound to procoagulant platelets, suggesting the Annexin V modification permits targeted delivery to sites of thrombosis. " 01/01/2003 - "To measure fibrino/thrombolysis release of FITC-labeled fibrinogen, added prior to thrombus formation, was measured over 24 h. " 08/01/2015 - "In vivo test of a classical acute pulmonary thromboembolism model in rat showed that when the injected dose was 5 mg/kg or above, FGFC1 was effective in dissolution of extrinsic FITC-fibrin induced blood clots. " 11/01/2003 - "During hemorrhagic shock and thrombosis, flow velocity and oxygen partial pressure of blood decreased in the brain and liver tissues that can be visualized by using FITC stained erythrocytes and Pd-porphyrin derivative as a pO(2) probe. "
5.	Breast Neoplasms (Breast Cancer) 03/20/2023 - "Multiphoton Microscopy of FITC-labelled Fusobacterium nucleatum in a Mouse in vivo Model of Breast Cancer." 01/01/2022 - "After breast cancer MDA-MB-231 cells were infected and irradiated, cellular proliferation and colony formation were measured, apoptotic rate was detected by FCM after AnnexinV-FITC/PI staining. " 08/12/2021 - "The fluorescent CNCs/FA-CS-FITC nanosystems with a rod-like morphology showed good stability in simulated physiological and non-physiological conditions and non-cytotoxicity towards MDA-MB-231 breast cancer cells. " 09/01/2020 - "The clinical application of FITC/fluorescence nanoparticle tagged RVV-NGFa for the ex vivo and in vivo diagnosis of breast cancer is highly promising." 09/01/2020 - "The fluorescence (FITC)-tagged RVV-NGFa depicted a strong fluorescence signal that was observed under a fluorescence microscope and determined by fluorescence microplate reader assay post binding to breast cancer cells; but no fluorescence signal was detected after incubating FITC-RVV-NGFa with non-cancerous L6 and HEK-293 cells. "

Related Drugs and Biologics

1.	Fluorescein (Funduscein)
2.	isothiocyanic acid
3.	Dextrans (Dextran)
4.	fluorescein isothiocyanate dextran
5.	Annexin A5
6.	Peptides (Polypeptides)
7.	Lectins
8.	Ligands
9.	Monoclonal Antibodies
10.	Coloring Agents (Dyes)

Related Therapies and Procedures

1.	Therapeutics
2.	Lasers (Laser)
3.	Drug Therapy (Chemotherapy)
4.	Aftercare (After-Treatment)
5.	Quantum Dots (Quantum Dot)