RNA Probes

RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.

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Networked: 92 relevant articles (3 outcomes, 10 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Järveläinen, Hannu: 2 articles (01/2013 - 01/2013)
2.	Sainio, Annele: 2 articles (01/2013 - 01/2013)
3.	Boström, Pia: 2 articles (01/2013 - 01/2013)
4.	Panda, M: 1 article (09/2015)
5.	Padhy, R N: 1 article (09/2015)
6.	Rath, S: 1 article (09/2015)
7.	Sahu, M C: 1 article (09/2015)
8.	Hur, Sung-Pyo: 1 article (08/2014)
9.	Sawada, Yuji: 1 article (08/2014)
10.	Takemura, Akihiro: 1 article (08/2014)

Related Diseases

1.	Infection 03/01/1996 - "The in situ hybridization technique with radiolabeled strand-specific RNA probes was used to determine target cells of virus infection and virus replication. " 09/01/1995 - "Strand-specific RNA probes and PCR were used to indicate the presence of the virus in various parts of infected banana plants during infection on the basis of dsDNA replicative intermediates of BBTV. " 07/01/1994 - "Chemiluminescence molecular hybridization includes the use of RNA probes which, on the basis the identification of different viral strains, allow a subdivision of infections into low and high/medium risk. " 12/01/1990 - "Analysis of RNA stability and (-) strand content in viral infections using biotinylated RNA probes." 08/01/1990 - "In this report we demonstrate that single-stranded RNA probes can be successfully used in an in situ hybridization assay to quantify viral expression during this persistent infection. "
2.	Dengue (Dengue Fever) 01/01/1994 - "With these RNA-probes we have been able to detect molecules of serotype-specific dengue 4 viral RNA. " 12/01/1990 - "By 1983 we had sensitive isolation systems including arthropods and arthropod cell cultures, specific-and group-reactive monoclonal antibodies, and a rapid and sensitive immunoassay for IgM and IgG. Progress in the past 7 years encompasses the use of monocyte lines for primary isolation, a more rapid plaque reduction neutralization test with BHK-21 cells, labeled RNA probes to detect dengue-specific nucleic acid, and improved ELISA technology with specific synthetic and engineered antigens."
3.	Hemorrhagic Fever with Renal Syndrome (HFRS) 01/01/1987 - "Radiolabeled RNA probes generated from cDNA clones of the M and S genome segments of Hantaan virus readily detected Hantaan virus and two isolates from Korean hemorrhagic fever patients but were less effective in detecting four other hantaviruses."
4.	Edema 12/01/1991 - "Non-radioactive in situ hybridization of formalin-fixed paraffin-embedded placental and foetal tissue, using virus-specific DNA or RNA probes, may be helpful for the diagnosis of foetal virus infection causing foetal hydrops, granulomatous placentitis and abortion. "
5.	Squamous Cell Carcinoma (Epidermoid Carcinoma) 10/01/1990 - "Normal tongue and cervical mucosa, premalignant cervical and vulvar lesions, primaries and metastases of squamous cell carcinomas from the oral, laryngeal, cervical and vulvar mucosa were analyzed for c-erbB2 and c-myc transcription with northern-blots using 32P single-stranded RNA probes. " 10/01/1999 - "This paper describes a study of squamous intraepithelial lesions (SILs, n=36), squamous cell carcinomas (SCCs, n=42), and normal mucosa (n=5) of the uterine cervix by in situ hybridization with (35)S-labelled RNA probes. " 04/01/1993 - "We used in situ hybridization with 35S-labeled RNA probes and immunohistochemistry to localize cellular sites of 92-kd gelatinase production in sections of invasive squamous cell carcinoma. " 05/01/1992 - "In the present study, c-myc overexpression was studied in ten carcinomas of different origin (6 mammary adenocarcinomas, 2 vulvar and 2 bronchial squamous cell carcinomas) by in situ hybridization (ISH) with 35S-labeled RNA probes and by immunohistochemistry (IHC). " 03/10/2003 - "These data were obtained with RNA probes pooled from several BCC of different donors and were subsequently confirmed by semiquantitative RT-PCR for Janus protein tyrosine kinase 3 (Jak3), microsomal glutathione S-transferase 1 (GST 12), teratocarcinoma-derived growth factor cripto, glutaredoxin and the monocyte chemoattractant protein 1 (MCP-1) in 10 individual BCC specimens, 2 squamous cell carcinoma (SCC), the cell line HaCaT and cultured normal human keratinocytes (NHK) in comparison to normal skin. "

Related Drugs and Biologics

1.	Paraffin
2.	Formaldehyde (Formol)
3.	DNA (Deoxyribonucleic Acid)
4.	Complementary DNA (cDNA)
5.	Monoclonal Antibodies
6.	Antigens
7.	Immunoglobulin M (IgM)
8.	Immunoglobulin G (IgG)
9.	Messenger RNA (mRNA)
10.	RNA (Ribonucleic Acid)

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4.	Lasers (Laser)