RNA Probes

RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.

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Networked: 171 relevant articles (3 outcomes, 16 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Beechem, Joseph M: 2 articles (01/2020 - 01/2020)
2.	Boström, Pia: 2 articles (01/2013 - 01/2013)
3.	Järveläinen, Hannu: 2 articles (01/2013 - 01/2013)
4.	Sainio, Annele: 2 articles (01/2013 - 01/2013)
5.	Nakayama, Jun: 2 articles (05/2007 - 01/2006)
6.	Ahmad, Meleha T: 1 article (06/2022)
7.	Campbell, Ashley A: 1 article (06/2022)
8.	Daoud, Yassine: 1 article (06/2022)
9.	Eberhart, Charles G: 1 article (06/2022)
10.	Mahoney, Nicholas: 1 article (06/2022)

Related Diseases

1.	Virus Diseases (Viral Diseases) 03/01/1996 - "The in situ hybridization technique with radiolabeled strand-specific RNA probes was used to determine target cells of virus infection and virus replication. " 12/01/1990 - "Analysis of RNA stability and (-) strand content in viral infections using biotinylated RNA probes." 06/01/2002 - "In attempts to gain insights into the mechanism of PRRSV persistence in boars, tissue distribution and sites of viral infection were investigated by in situ hybridization (ISH) using digoxigenin-labeled RNA probe and the ISH results were compared with those of reverse transcription-nested polymerase chain reaction (RT-nested PCR). " 06/01/1991 - "[The use of DNA and RNA probes in the diagnosis of human viral diseases (a review of the literature)]." 12/01/1991 - "Non-radioactive in situ hybridization of formalin-fixed paraffin-embedded placental and foetal tissue, using virus-specific DNA or RNA probes, may be helpful for the diagnosis of foetal virus infection causing foetal hydrops, granulomatous placentitis and abortion. "
2.	Dengue (Dengue Fever) 01/01/1994 - "With these RNA-probes we have been able to detect molecules of serotype-specific dengue 4 viral RNA. " 12/01/1990 - "By 1983 we had sensitive isolation systems including arthropods and arthropod cell cultures, specific-and group-reactive monoclonal antibodies, and a rapid and sensitive immunoassay for IgM and IgG. Progress in the past 7 years encompasses the use of monocyte lines for primary isolation, a more rapid plaque reduction neutralization test with BHK-21 cells, labeled RNA probes to detect dengue-specific nucleic acid, and improved ELISA technology with specific synthetic and engineered antigens."
3.	Hemorrhagic Fever with Renal Syndrome (HFRS) 01/01/1987 - "Radiolabeled RNA probes generated from cDNA clones of the M and S genome segments of Hantaan virus readily detected Hantaan virus and two isolates from Korean hemorrhagic fever patients but were less effective in detecting four other hantaviruses."
4.	Edema (Dropsy) 12/01/1991 - "Non-radioactive in situ hybridization of formalin-fixed paraffin-embedded placental and foetal tissue, using virus-specific DNA or RNA probes, may be helpful for the diagnosis of foetal virus infection causing foetal hydrops, granulomatous placentitis and abortion. "
5.	Infections 09/01/1995 - "Strand-specific RNA probes and PCR were used to indicate the presence of the virus in various parts of infected banana plants during infection on the basis of dsDNA replicative intermediates of BBTV. " 10/01/1994 - "Dermacentor andersoni ticks taken from the infected animal were tested for infection by RNA probe analysis. " 07/01/1994 - "Chemiluminescence molecular hybridization includes the use of RNA probes which, on the basis the identification of different viral strains, allow a subdivision of infections into low and high/medium risk. " 02/01/1991 - "In situ hybridization with enterovirus-specific and highly sensitive single-stranded RNA probes is of particular interest for the diagnosis of myocardial infections and for studies concerning viral RNA replication." 10/01/2001 - "Northern blot analysis of RNA from BHV1-infected cells with a single strand RNA probe complementary to UL12 detected four specific 3' coterminal viral transcripts of 4.2, 3.7, 2.2, and 0.7 kb that accumulated simultaneously from 6 to 24 hours post-infection (p.i.). "

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1.	Paraffin
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5.	Complementary DNA (cDNA)
6.	Antigens
7.	Monoclonal Antibodies
8.	Immunoglobulin M (IgM)
9.	Immunoglobulin G (IgG)
10.	Messenger RNA (mRNA)

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